人牙髓细胞体外培养及向成牙本质细胞诱导分化

目的利用组织块酶解法体外培养人牙髓细胞并进行鉴定。方法通过组织块酶解法行人牙髓细胞体外培养,免疫细胞化学法鉴定细胞组织来源,测定体外复层生长的人牙髓细胞碱性磷酸酶活性,并检测其诱导矿化能力。结果牙髓细胞在连续培养后具有复层生长特性,并具有显著的碱性磷酸酶活性,连续培养的牙髓细胞可形成矿化结节。结论建立了成牙本质细胞样细胞体外培养体系,所获得的成牙本质细胞样细胞具有成牙本质细胞的部分形态和生物学功能。

STUDY ON ENZYMATICALLY RELEASED HUMAN DENTAL PULP CELLS WITH COLLAGENASE I IN VITRO

Objective To investigate the alkaline phosphatase activity of enzymatically released human denial pulp cells with eollagenase I in vitro. Methods We cultivated human denial pulp cells from connective tissue explants digested with eollagenase I Immunocytochemieal staining was performed for characterization. When the subcultured cells grew in multilayers, the activity of alkaline phosphatase was examined and the ability of mineralization was detected. Results Cultured cells were all vimentin positive and keratin negative staining. ALP activity was up - regulated after mineralization compared with control. Conclusion These results suggest that human dental pulp cells can be cultivated preferably from tissue explants digested with eollagenase I in vitro. Enzyme histochemistry is useful for studying the biological behavior of dental pulp cells.