| 高产GL-7ACA酰化酶菌株的获得及其培养条件的研究 |
| |
| 引用本文： | 刘光荣,张捷,李文筠,许承寿,袁剑萍,陈俊琳,张跃年,虞和慈,呼庭华. 高产GL-7ACA酰化酶菌株的获得及其培养条件的研究[J]. 中国抗生素杂志, 1993, 0(6) |
| |
| 作者姓名： | 刘光荣 张捷 李文筠 许承寿 袁剑萍 陈俊琳 张跃年 虞和慈 呼庭华 |
| |
| 作者单位： | 中国科学院上海生物工程研究中心 上海(刘光荣)，上海第三制药厂 上海200052(张捷，李文筠，许承寿，袁剑萍，陈俊琳，张跃年，虞和慈)，上海第三制药厂 上海200052(呼庭华) |
| |
| 摘 要： | 从假单胞菌的野生菌株SP-821出发,经化学、物理诱变,获得GL-7ACA酰化酶高产菌株79u-18,酶活性高达700u/L。在菌株选育过程中一个突破性进展是分离获得构成型GL-7ACA酰化酶产生菌株,即该菌在培养中不需加入戊二酸作诱导剂。本文介绍上述菌株的获得及培养条件的研究。
|
| 关 键 词： | 假单孢菌GL-7ACA酰化酶 诱导型酶 构成型酶 GL-7ACA 7-ACA |
STUDIES ON THE SELECTION OF HIGH-YIELD GL-7ACA-ACYLASE PRODUCER, PSEUDOMONAS 79U-18 AND ITS CULTURAL CHARACTERISTICS |
| |
| Liu Guang-rong,Zhang Jie,Li Wen-jun,Xu Yong-shou,Yuan Jian-ping,Chen Jun-lin,Zhang Yue-nian,Yu He-ci and Hu Ting-hua. STUDIES ON THE SELECTION OF HIGH-YIELD GL-7ACA-ACYLASE PRODUCER, PSEUDOMONAS 79U-18 AND ITS CULTURAL CHARACTERISTICS[J]. Chinese Journal of Antibiotics, 1993, 0(6) |
| |
| Authors: | Liu Guang-rong Zhang Jie Li Wen-jun Xu Yong-shou Yuan Jian-ping Chen Jun-lin Zhang Yue-nian Yu He-ci Hu Ting-hua |
| |
| Abstract: | By treating the parent strain, wild type of Pseudomonas SP-821, with a series of Physico-chemical mutagenic agents, a high-yield GL-7ACA-acylase producer, Pseudomonas 79u-18 which specific activity is as high as 700 u/L was isolated.In the course of the strain selection studies, a breakthrough result was obtained on the isolation of constitutive type of GL-7ACA-acylase enzyme producer which was the transformed type of the preceding enzyme, glutaric inducing one.Detail of the selection experiment procedure as well as the fermentation conditions were reported. |
| |
| Keywords: | Pseudomonas GL-7ACA-acylase Inducing enzyme Constitutive enzyme GL-7ACA 7-ACA |
| 本文献已被 CNKI 等数据库收录！ |
|
