| 异氟醚预处理对离体神经元损伤模型的作用 |
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| 引用本文： | 曾真,李颖川,张晓峰,焦志华,张昕. 异氟醚预处理对离体神经元损伤模型的作用[J]. 中国临床医学, 2009, 16(2): 291-293 |
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| 作者姓名： | 曾真 李颖川 张晓峰 焦志华 张昕 |
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| 作者单位： | 上海交通大学附属第六人民医院麻醉科,上海,200233 |
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| 摘 要： | 目的：观察异氟醚对氧糖剥夺离体神经元损伤的保护作用。方法：新生小鼠神经元原代培养14d后,造成氧糖剥夺离体神经元缺血模型,4h后复氧、复糖,模拟再灌注模型。随机分为3组：C组为对照组;A组用1MAC异氟醚通入培养液预处理1h;B组用2MAC异氟醚通入培养液预处理1h。各组分别于复氧复糖后4、12和24h取神经元细胞计数后作四唑盐（MTT）比色试验。并取复氧、复糖24h各组神经元细胞做台盼蓝染色,计阳性细胞数百分比。另取原代培养神经元,造成氧糖剥夺离体神经元缺血模型30min后分成两组后分别按C组和A组处理,复氧、复糖4h后,免疫组化SABC法染色,比较nNOS和iNOS活性。结果：复氧、复糖后A组和B组MTT各时点值均大于C组,且台盼蓝染色阳性细胞数百分比A组和B组均小于C组,但A组和B组间无显著差异。免疫组化染色结果显示：A组nNOS和iNOS的表达灰度值均低于C组。结论：异氟醚能减轻氧糖剥夺离体神经元的损伤,抑制神经元nNOS和iNOS活性是其可能机制之一。
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| 关 键 词： | 异氟醚 神经元 氧糖剥夺 |
The Effect of Isoflurane on the Oxygen and Glucose Deprivation in Cultured Neuronal Cell |
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| ZENG Zhen,LI Yingchuan,ZHANG Xiaofeng,JIAO Zhihua,ZHANG Xin. The Effect of Isoflurane on the Oxygen and Glucose Deprivation in Cultured Neuronal Cell[J]. Chinese Journal Of Clinical Medicine, 2009, 16(2): 291-293 |
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| Authors: | ZENG Zhen LI Yingchuan ZHANG Xiaofeng JIAO Zhihua ZHANG Xin |
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| Affiliation: | ZENG Zhen LI Yingchuan ZHANG Xiaofeng JIAO Zhihua ZHANG Xin Department of Anesthesiology,The 6th People's Hospital,Jiaotong University,Shanghai 200233 |
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| Abstract: | Objective:To investigate the protective effect of isoflurane on the oxygen and glucose deprivation(OGD) in cultured neuronal cell. Methods: The cultured neurons dissociated from the mouse brain were observed on the 14th day after cultured. To make the ischemia-reperfusion model in vitro, the neurons were exposed to OGD 4h before they were placed to nomal culture media. Group Control was taken as blank group. In group A, 1 MAC isoflurane was given to neurons in the culture media before the oxygen-glucose(OG)... |
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| Keywords: | Isofurane Neuron Oxygen and glucose deprivation |
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