米非司酮对卵巢颗粒细胞中Caspase-3蛋白表达的影响

目的探讨米非司酮对卵巢颗粒细胞中Caspase-3蛋白表达的影响。方法选用雌性未孕Sprague-Dawley大鼠40只,随机分为米非司酮低剂量组、米非司酮中剂量组、米非司酮高剂量组及空白对照组,于动情间期早期开始分别予米非司酮1.04mg/(kg.d)、2.604mg/(kg.d)、10.4mg/(kg.d)灌胃,连续4周。用药结束时处死动物,免疫组化检测Caspase-3蛋白在卵巢颗粒细胞中的表达。结果各级生长卵泡的颗粒细胞中均有Caspase-3蛋白表达,对照组颗粒细胞胞浆呈浅棕黄色,低剂量组、中剂量组各级生长卵泡中均可见颗粒细胞胞浆呈棕黄色,高剂量组绝大多数颗粒细胞胞浆呈棕黄至棕褐色,部分出现胞核阳性表达。随着用药剂量增加,Caspase-3蛋白表达增强,积分光密度值呈上升趋势,差异具有统计学意义(P<0.05)。结论调节Caspase-3蛋白表达可能是米非司酮诱导颗粒细胞凋亡的途径之一。

The Expression of Caspase-3 in Granulosa Cells of Endometriosis Treated with Mifepristone

Objective To investigate the effect of mifepristone on the expression of Caspase-3 in the granulosa cells.Methods Forty Sprague-Dawley(SD) female rats with(230±20) g weight were divided into four groups,low-dose group with 1.04 mg/(kg·d) of mifepristone,middle-dose group with 2.604 mg/(kg·d)of mifepristone,high dose group with 10.4 mg/(kg·d)of mifepristone,as well as blank group.Mifepristone tablets were given through gastromy in diestrus of rat for four weeks.Rats were sacrificed after the treatment,and the the expression of Caspase-3 in the granulosa cells of developing follicles was detected by immunohistochemical staining.Results The Caspase-3 protein expression was observed in granulosa cells of developing follicles,while the positive expression level and integrated optical density(IOD) value were increased along with the dosage of mifepristone increasing.The difference among the three dosage groups were significant(P<0.05).Conclusion Regulating Caspase-3 protein expression may be one of the ways for mifepristone inducing granulosa cell apoptosis.