rhBNP对心肌梗死后心肌细胞病理学和细胞凋亡的研究

目的:观察rhBNP对大鼠心肌梗死后心肌细胞病理学和细胞凋亡的影响。方法:建立大鼠心肌梗死模型,分为MI对照组和BNP治疗组。治疗组给予rhBNP连续静滴4周,治疗48 h、1、2、3和4周时处死大鼠,进行心肌组织切片HE染色,TUNEL法测心肌细胞凋亡指数MAI,免疫组化测Fas与FasL蛋白阳性染色指数。结果:治疗组MAI和Fas/Fasl蛋白阳性率与对照组比较下降有显著性差异(P<0.05)。结论:连续给予rhBNP能够通过减少MI后心肌细胞凋亡保护缺血心肌,达到改善心功能的目的。

Effects of recombinant human brain natriuretic peptide on myocardial pathology and apoptosis after myocardial infarction in rats

Objective:To assess the effect of long-term infusion of recombinant human brain natriuretic peptide(rhBNP) on myocardial pathology and apoptosis after myocardial infarction(MI) in rats.Methods: All rats were divided into rhBNP treatment group and MI control group.The MI rats in treatment group were treated by intravenous rhBNP injection(4 weeks).Myocardial tissues of rats were obtained after treatment of 48 hours,1,2,3 and 4 weeks.The tissue sections were stained with hematoxylin and eosin(HE).Myocardial apoptotic index(MAI) was determined by TUNEL and Fas/FasL protein was detected by immunohistochemical method.Results: Both MAI and Fas/FasL protein in treatment group were significantly reduced compared with control group(P<0.05).Conclusion: Continuous rhBNP injection may effectively protect myocardial cells after myocardial infarction through reducing myocardial apoptosis,which could improve cardiac function.