miR-21、miR-126、miR-143和miR-373在正常宫颈组织、宫颈癌组织及Hela细胞中的表达差异

目的研究miR-21、miR-126、miR-143和miR-373在正常宫颈组织、宫颈癌组织及Hela细胞中的表达差异,探讨microRNAs(miRNAs)对宫颈癌发生的调控作用。方法荧光实时定量检测20例良性肿瘤患者的正常宫颈组织,20例宫颈癌组织及Hela细胞中miR-21、miR-126、miR-143和miR-373的表达。结果 miR-21在宫颈癌组织及Hela细胞系中高表达,在正常宫颈标本中低表达,在宫颈癌组织中相对定量为正常宫颈组织的11.3196倍(P<0.05);miR-143、miR-373在宫颈癌组织及Hela细胞中均低表达,在正常宫颈标本中高表达,miR-143、miR-373在宫颈癌组织中相对定量分别为正常宫颈组织的0.1553倍和0.4907倍(P<0.05);miR-126的表达无明显变化。结论 miRNAs与宫颈癌的发生和调控密切相关。在宫颈癌组织和Hela细胞中,miR-21表达上调,可能在宫颈癌的发生过程中发挥癌基因的作用;miR-143、miR-373表达下调可能发挥抑癌基因的作用;miR-126表达无差异,与宫颈癌无明显关系。

Differential Expression of miR-21,miR-126,miR-143,miR-373 in Normal Cervical Tissue,Cervical Cancer Tissue and Hela Cell

Objective To investigate the differential expression of miR-21,miR-126,miR-143 and miR-373 in normal cervical tissue,cervical cancer tissue and Hela cell.Methods The expressions of miR-21,miR-126,miR-143 and miR-373 were detected by real-time PCR in cervical cancer tissue,cervical tissue of benign uterine tumor and Hela cell.Results High expression of miR-21 was observed in cervical cancer and Hela cell,while low expression was observed in normal cervical tissue.The relative quantification of miR-21 in cerveical cancer was 11.3196 times that of miR-21 in normal cervical tissue(P<0.05).The expression levels of miR-143 and miR-373 in cervical cancer and Hela cell were lower than those of normal cervical tissue.The relative quantification of miR-143 in cerveical cancer was 0.1553 times that of normal cervical tissue(P<0.05),and the relative quantification of miR-373 in cerveical cancer was 0.4907 times that of normal cervical tissue(P<0.05).The expression of miR-126 had no significant difference among cervical cancer tissue,Hela cell and normal cervical tissue(P>0.05).Conclusion miRNAs are closely related to the occurrence and regulation of cervical cancer.The high expression of miR-21 in cervical cancer and Hela cell indicate that it may play a possible role of oncogenes,while miR-143 and miR-373 with low expression may play the role of tumor suppressor genes.