川芎嗪预处理对大鼠肝缺血再灌注损伤能量代谢的保护作用

目的探讨川芎嗪预处理对大鼠肝脏缺血再灌注损伤(IRI)后肝脏能量代谢的影响。方法将120只雄性Wistar大鼠随机分为假手术组、实验组和对照组3组,实验组和对照组制作70%大鼠肝脏IRI模型,实验组大鼠在IRI前3d,每日腹腔注射川芎嗪注射液2mL,各组大鼠分别于术后1h、6h、24h、72h取肝组织观察组织形态学变化,检测肝细胞线粒体呼吸控制率(RCR)、磷氧比(P/O)和肝组织三磷酸腺苷(ATP)含量变化。结果①术后各个相应时相点实验组肝组织病理损伤均较对照组轻;②术后1h、6h、24h对照组肝细胞线粒体RCR及P/O均低于实验组(P均<0.05),72h均恢复正常(P>0.05);③实验组术后各相应时相点肝细胞ATP含量均高于对照组(P<0.05),并且恢复正常也比对照组较早。结论川芎嗪预处理能够改善肝脏能量代谢,提高缺血再灌注后肝脏的抗损伤能力。

Protective Effect of Ligustrazine Preconditioning on Hepatic Energy Metabolism in Ischemia/Reperfusion Injury of Rat Liver

Objective Objective To investigate the protective effects of Ligustrazine preconditioning against hepatic ischemia/reperfusion injury(IRI) in rats.Methods Fifty male Wistar rats were randomly allocated into 3 groups: sham operation group,in which animals underwent laparotomy,experimental group and control group in which were treated with 70% IRI of the liver,especially,the animals in experimental group was given intraperitoneal injection of 2 mL Ligustrazine per day for 3 days before operation.After the operation,liver tissues were harvested at 1 h,6 h,24 h and 72 h for the study of histomorphological change,the respiratory control ratio(RCR) and phosphorus:oxygen ratio(P/O) of hepatocytes mitochondria,and the contents of adenosine triphosphate(ATP) of liver tissue.Results ①The damage hepatic tissue in experimental group was slighter than that in control group at each corresponding time-point after operation.②The RCR and P/O ratio at each corresponding time-point were higher in experimental group than those in control group(P<0.05),and all of the two groups recovered after 72 h.③The ATP concentration in experimental group also was higher than that in control group at each corresponding time-point,and recovered faster than control group.Conclusion The current results show that Ligustrazine preconditioning may improve energy metabolism of rat liver in ischemia reperfusion injury.