聚羟基丁酸与羟基辛酸骨组织工程支架的表面修饰**◆

目的：研究多聚赖氨酸表面修饰的聚羟基丁酸与羟基辛酸骨组织工程支架对细胞黏附、增殖及分化的影响。方法：冷冻干燥/颗粒沥滤法制备聚羟基丁酸与羟基辛酸多孔支架，将其置于0.1，1.0，10 g/L的多聚赖氨酸溶液中，负压排气吸附进行表面修饰。灭菌后的支架置于48孔板，接种兔骨髓间充质干细胞。分别于1，4，7，10，14 d取样。结果：3个多聚赖氨酸修饰组的细胞黏附率均高于支架-细胞组(P < 0.01)，且细胞黏附率随多聚赖氨酸修饰质量浓度的增大而显著提高。1，4 d时10 g/L多聚赖氨酸修饰组的细胞增殖活性和碱性磷酸酶活性均高于其他两组，但至10，14 d时却低于其他两组。结论：多聚赖氨酸修饰的聚羟基丁酸与羟基辛酸多孔支架有利于提高骨髓间充质干细胞的黏附率，但1.0 g/L的多聚赖氨酸修饰更有利于细胞的增殖与促成骨分化。关键词：聚羟基丁酸与羟基辛酸；骨组织工程；支架；表面修饰

Surface modification of poly (hydroxybutyrate-co-hydroxyoctanoate) tissue engineering scaffolds

OBJECTIVE: To investigate the effect of poly (hydroxybutyrate-co-hydroxyoctanoate) (PHBHOx) bone engineering scaffold modified by poly-L-lysine (PLL) on cell adhesion, proliferation, and differentiation. METHODS: Porous PHBHOx scaffolds were prepared by freeze-drying/particle leaching method. Then, the scaffolds were placed into 0.1, 1.0, and 10 g/L PLL solution and performed with negative pressure exhaust. The modified scaffolds were autoclaved and then inoculated with rabbit bone marrow mesenchymal stem cells (BMSCs) in a 48-well plate. Cells were sampled at days 1, 4, 7, 10, and 14, respectively. RESULTS: Cell adhesion rate was significantly greater in the three PLL-modified scaffold-cell groups than in the scaffold-cell group (P < 0.01) and increased with the increased concentration of PLL modification. Cell proliferation and ALP activity in the 10 g/L PLL-modified scaffold-cell group were significantly higher than other two groups at days 1 and 4 but was lower at days 10 and 14. CONCLUSION: In accordance with the results mentioned above, porous PHBHOx tissue engineering scaffolds modified by PLL were beneficial to improve the adhesion rate of BMSCs, while 1.0 g/L PLL was more beneficial for cell proliferation and osteogenic differentiation.