Induction of a Th17 cell response by Helicobacter pylori Urease subunit B |
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Authors: | Zhang Jin-Yu Liu Tao Guo Hong Liu Xiao-Fei Zhuang Yuan Yu Shu Chen Li Wu Chao Zhao Zhuo Tang Bin Luo Ping Mao Xu-Hu Guo Gang Shi Yun Zou Quan-Ming |
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Institution: | a Department of Clinical Microbiology and Immunology, College of Medical Laboratory, The Third Military Medical University, Chongqing 400038, PR China b Department of Gastroenterology, Xinqiao Hospital, The Third Military Medical University, Chongqing 400038, PR China |
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Abstract: | Th17 cells represent a novel subset of CD4+ T cells, which is associated with Helicobacter pylori infection. In the present study, we investigated the potential role of Urease subunit B (UreB) in the induction of Th17 cell response. Co-cultured splenic lymphocytes from H. pylori-infected mice with the recombinant UreB (rUreB) elevated IL-17 secretion and caused an increase in the number of Th17 cells. The expression of IL-6 and IL-23 p19 was significantly increased in rUreB-stimulated macrophages. Whole cell protein (WCP) of UreB-deficient strain (UreB− strain) induced less Th17 cell responses than that of wild-type strain. In addition, subcutaneous and intranasal immunization of rUreB elicited antigen-specific Th17 cell responses. Intranasal immunization of rUreB reduced H. pylori colonization in the stomach, which was closely related with the increased rUreB-specific Th17 cell responses. These results suggest that UreB is an important protein which is able to elicit Th17 cell responses against H. pylori both in vivo and in vitro. |
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Keywords: | IFN-γ interferon-γ PMA phorbol 12-myristate 13-acetate IL interleukin TGF-β transforming growth factor-β |
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