| Cold—preserved human spermatozoa in electrolyte—free solution retain their penetration capacity |
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| 引用本文： | 全松,周海宽,山野修司,中坂尚代,青野敏博. Cold—preserved human spermatozoa in electrolyte—free solution retain their penetration capacity[J]. 中国人民解放军军医大学学报, 2003, 18(1): 51-55 |
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| 作者姓名： | 全松 周海宽 山野修司 中坂尚代 青野敏博 |
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| 作者单位： | Department of Obstetrics & Gynecology，Nanfang Hospital，First Military Medical University，Department of Urological Surgery，Nanfang Hospital，First Military Medical University，Department of Obstetrics & Gynecology，School of Medicine，the University of Tokushima，Department of Obstetrics & Gynecology，School of Medicine，the University of Tokushima，Department of Obstetrics & Gynecology，School of Medicine，the University of Tokushima Guangzhou，510515，Guangzhou，510515，Tokushima 770，Japan，Tokushima 770，Japan，Tokushima 770，Japan |
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| 摘 要： | Objective: To evaluate penetration capacity of human sperm preserved in electrolyte-free (EF) solution at 4 ℃. Methods: The motility, acrosomal status penetration rate and fertility index of human sperm were assessed before and after cold-preservation in EF solution, respectively. Results: The motility of human sperm cold-preserved in EF solution for 1 week was significantly higher than that of human sperm cold-preserved in modified human tubal fluid ( mHTF) (43. 4% ± 7. 9% vs 9. 5% ±2. 5% , P <0. 01 ). Although acrosomal status of human sperm cold-preserved in the EF solution before reinitiation was not different from those of the fresh sperm ( capacitated sperm; 7. 6% ± 1. 8% vs6.4±1.8%; acrosome-reacted sperm; 3.0% ±1.7% vs2.4±1.1%, P>0.05), the percentage of capacitated and acrosome-reacted sperm in the EF solution significantly increased after reinitiation (capacitated sperm; 16. 0% ± 2.3%vs7.6±1.8%, acrosome-reacted sperm: 9. 4% ±2.1% vs 3. 0% ±1.7%, P<0.01). The penetration rate and fe
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| 关 键 词： | 人精子 冷藏 冷冻保存 电解质 EF溶液 精子获能 顶体反应 穿透能力 |
Cold-preserved human spermatozoa in electrolyte-free solution retain their penetration capacity |
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| QUAN Song,ZHOU Hai-kuan,Shuji Yamano,Hisayo Nakasaka Toshihiro Aono. Cold-preserved human spermatozoa in electrolyte-free solution retain their penetration capacity[J]. Journal of Medical Colleges of PLA(China), 2003, 18(1): 51-55 |
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| Authors: | QUAN Song ZHOU Hai-kuan Shuji Yamano Hisayo Nakasaka Toshihiro Aono |
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| Affiliation: | QUAN Song,ZHOU Hai-kuan,Shuji Yamano,Hisayo Nakasaka Toshihiro Aono Department of Obstetrics & Gjnecology,Nanfang Hospital,First Military Medical University,Guangzhou,510515 Department of Urological Surgery,Nanfang Hospital,First Military Medical University,Guangzhou,510515 Department of Obstetrics & Gynecology,School of Medicine,the University of Tokushima,Tokushima 770,Japan |
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| Abstract: | |
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| Keywords: | sperm electrolyte-free solution cold-preservation capacitation acrosome reaction penetration capacity |
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