| 合成肽制备乙型肝炎病毒前S_1抗体 |
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| 引用本文： | 徐永耀,俞翠珠,候杰,张维,李平洋,闻玉梅. 合成肽制备乙型肝炎病毒前S_1抗体[J]. 中国免疫学杂志, 1990, 0(2) |
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| 作者姓名： | 徐永耀 俞翠珠 候杰 张维 李平洋 闻玉梅 |
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| 作者单位： | 上海医科大学微生物教研室，上海市医学化验所，上海市医学化验所，上海医科大学微生物教研室，上海医科大学微生物教研室，上海医科大学微生物教研室 上海，上海，上海，上海 |
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| 摘 要： | 根据乙型肝炎病毒前S_1蛋白中的第21～47氨基酸决定簇人工合成肽P21～47。将P21～47与破伤风类毒素偶联成免疫原,免疫家兔获得了抗体。该抗体不仅可与P 21～47反应,还可与乙型肝炎病毒的PreS_1抗原发生反应。鉴于PreS_1与乙型肝炎病毒吸附于靶细胞表面受体有关,本抗体不仅可用于检测PreS_1抗原,还可研究病毒与受体间相互关系。
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| 关 键 词： | 乙型肝炎病毒 合成肽 PreS_1抗体 决定簇 |
Using synthetic peptide to generate to specific antibody against hehatitis B virus protein Pre-S(?) |
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| Xu Yongyao et al. Using synthetic peptide to generate to specific antibody against hehatitis B virus protein Pre-S(?)[J]. Chinese Journal of Immunology, 1990, 0(2) |
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| Authors: | Xu Yongyao et al |
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| Affiliation: | Xu Yongyao et al Department of Microbiology,Shanghai Medical University,Shanghai,China 200032 |
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| Abstract: | A peptide was synthesized based on the 2l-47 amino acid sequence of Hepatitis B Virus PreSl protein. This peptide (p21-47) was cross-linked to tetanus toxoid and used to immunize rabbit. Rabbit P21-47 not only reacted with the peptide but also reacted with preSl (Large portein of HBV envelope) in Western blotting. Since preSl seems to be involved in attachment of HBV to target cells, this anti P21-47 has the potentiality in studying virus-receptor intereaction as well as for assaying PreS (?)antigen. |
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| Keywords: | Hepatitis B virus Synthetic peptide Anti-PreS1 Epitopes |
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