荧光染料双染法观察汉防己对胸腺细胞的作用

目的观察防己（Radix Stephaniae Tetrandrae）对小鼠胸腺细胞增殖的影响。方法取昆明种小鼠的胸腺细胞，分为对照组和药物组，药物组用100μg／ml汉防己煎剂对胸腺细胞分别作用1h、6h、12h、24h后，采用DNA荧光染料双染法观察胸腺细胞增殖的影响。结果实验组可见典型凋亡细胞，对照组细胞大小比较均一，无明显形态学变化。随着药物作用时间的延长，凋亡小体的比例显著增加。结论防己可以诱导小鼠胸腺的细胞的凋亡。

Effect of Radix Stephaniae Tetrandrae on the Thymocyte by Double Fluorescent Staining

Objective To investigate the effect of radix stephaniae tetrandrae on the thymocyte by double fluorescent staining. Methods The thymocytes cultured from Kunming mice were randomly divided into control group and drug group which treated with Radix Stephaniae Tetrandrae decoc- tion（100 μg/ml）. The morphological changes of thymocytes were observed by double fluorescent staining after treating 1 h, 6 h, 12 h and 24 h, respectively. Results Apoptotic bodies were ob- served in thymocytes of drug group,no significant changes were seen in control group; and the rate of apoptotic bodies increased with the action time. Conclusion The Radix Stephaniae Tetrandrae decoction could induce the apoptosis of mice thymocyte.