抗子宫内膜抗体对人早孕绒毛滋养细胞凋亡及FasL蛋白表达的影响

目的:探讨体外培养人早孕绒毛滋养层细胞在游离抗子宫内膜抗体(Em-Ab)环境中,细胞凋亡指数(apoptosis index,AI)及凋亡相关因子配体(factor associated suicide ligend,FasL)的表达。方法:培养经胰蛋白酶/DNA酶Ⅰ联合消化,通过Percoll细胞分离液纯化得到绒毛滋养细胞。用TUNEL法测定EmAb(+)血清处理组和EmAb(-)血清处理组绒毛滋养细胞培养0、48、72、96h时的细胞凋亡指数,同时用免疫细胞化学法测定FasL蛋白的表达。结果:EmAb(+)血清处理组于培养72、96h时间点测得的AI明显高于EmAb(-)血清处理组(P<0.01);FasL蛋白表达明显低于EmAb(-)血清处理组;而培养48h AI及FasL蛋白表达无显著差异。结论:绒毛滋养细胞在EmAb(+)环境下AI增加,FasL蛋白表达减少。

Effects of anti-endometrium antibody on AI and FasL expression of cultured human cytotrophoblast cells

Objective：To investigate apoplosis index（AI） and factor associated suicide ligend（FasL） expression of cultured human cytotrophoblast cells under free EmAb environment. Methods： Chorionic villi tissue of normal 6 - 8 week pregnancy were obtained. Cytotrophoblast ceils were collected by trypsin-DNase I digestion and Percoll gradient centrifugation. They were cultured under EmAb（ ＋ ） serum group and EmAb（-） serum group for 0,48,72,96h. AI and FasL expression was observed by TUNEL and immunohistochemistry respectively. Results： AI in EmAb（ ＋ ） serum group was significantly higher than that of EmAb（-） serum group（P 〈 0.05 ）. FasL concentrations in EmAb （ ＋ ） serum group were significantly lower than those of EmAb（-） serum group when measured at 72h,96h culture period. AI and FasL concentrations were not obviously different when measured at 48h culture period （ P 〉 0.05 ）. Conclusion： AI of cytotrophoblast cells increased and FasL expression decreased when they were cultured under free EmAb environment.