急性局灶性脑缺血后细胞凋亡的动态变化

目的：细胞凋亡是细胞死亡的一种形式，对缺血后梗塞灶的发生，发展一定的作用。故测定缺血脑区出现ＤＮＡ片断化的细胞数量变化及在缺血脑区的分布状况，以了解脑缺血的病理生理及寻求防治措施。方法：利用线栓法制成大脑中动脉缺血动物模型，测定脑组织ＳＯＤ，ＬＰＯ含量变化及凋亡细胞的密度变化；

Dynamic detection of nerve cells of rat's brain after experimental occluded middle cerebral artery

Purpose:Apoptosis describes a specific form of cell death,and may be play an important role in development of this ischemic.Methods:The objective of this present study is to make animal brain ischemic model with middle cerebral artery occlusion(MCAO)by nylon thread,then measure the numbers and anatomic distribution of nerve cells apoptosis, assay the SOD and LPO content in same ischemic tissue. Results:The apoptic of nerve cells were primarily locatized to the inner boundary zone of the ischemic infarction,were distributed in penumbra zone, but in the central portion of the lesion was the necrosis,SOD content was decreased and LPO content was markedly increased.Conclusion:The results indicated that the ability of brain tissue to clean free radical was depressed in the ischemic area, so apoptosis is major form of cell death in penumbra zone,may contribute to the development of infarction after MCAO.This suggests to inhibite the free radical would be more important for controlling the ischemic tissue after strok.