| 肝缺血再灌注对细胞周期的影响 |
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| 引用本文： | 肖建生,叶启发.肝缺血再灌注对细胞周期的影响[J].中国现代医学杂志,2002,12(8):17-19. |
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| 作者姓名： | 肖建生 叶启发 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院器官移植研究所,武汉,430030 |
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| 摘 要： | 目的 :观察肝缺血再灌注对细胞周期的影响 ,以探讨移植肝功能不良的可能原因。方法 :18只Wistar大鼠随机分成假手术组 (sham) ,缺血 1h再灌注 1h组 (IR1) ,缺血 1h再灌注 4h组 (IR2 )三组。利用肝原位部分缺血再灌注模型 ,用流式细胞检测技术定量测定各期细胞数 ,观察随灌注时间的延长细胞周期的变化。结果 :与对照组相比 ,IR1组肝组织的细胞周期S期比例降低 ,G2 /M期比例升高 ;IR4组G0 /G1期细胞比例增加 ,G2 /M期细胞比例明显减少。结论 :肝缺血再灌注能引起细胞周期的变化 ,使细胞的增殖和分裂处于抑制状态 ,从而影响细胞的再生和组织的修复
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| 关 键 词： | 肝 缺血再灌注 细胞周期 流式细胞术 |
| 修稿时间： | 2002年3月11日 |
EFFECTS OF HEPATIC ISCHEMIA/REPERFUSION ON CELL CYCLE IN RAT |
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| Xiao Jiansheng,Ye Qifa Research Institute of Organ Transplantation,Tongji Hospital,Tongji Medical College of Huazhong University of Science and Technology,Wuhan.EFFECTS OF HEPATIC ISCHEMIA/REPERFUSION ON CELL CYCLE IN RAT[J].China Journal of Modern Medicine,2002,12(8):17-19. |
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| Authors: | Xiao Jiansheng Ye Qifa Research Institute of Organ Transplantation Tongji Hospital Tongji Medical College of Huazhong University of Science and Technology Wuhan |
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| Institution: | Xiao Jiansheng,Ye Qifa Research Institute of Organ Transplantation,Tongji Hospital,Tongji Medical College of Huazhong University of Science and Technology,Wuhan 430030 |
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| Abstract: | Objective:To observe the effects of hepatic ischemia reperfusion on cell cycle, and to investigate the probable causes of the poor liver function after transplantation. Methods: Eighteen Wistar rats were randomly divided into three groups: Sham operation group (sham group); Ischemia one hour reperfusion one hour group (IR1);Ischemia one hour reperfusion four hours group (IR2). The model of partial liver ischemia/reperfusion was used.The number of cells in each phase was measured by flow cytometry to observe changes in cell cycle as time of reperfusion extending.Results:Compared with sham group, S-phase ratio decreased and G 2/M-phase ratio increased in IR1 group; G 0/G 1-phase ratio increased and G 2 /M-phase ratio decreased significantly in IR2 group.Conclusion:Hepatic ischemia/reperfusion changed cell cycle, cell proliferation and split in rat liver were in restrained state that would influence cell regeneration and repair of tissue. |
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| Keywords: | Liver Ischemia Reperfusion Cell Cycle Flow Cytometric |
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