新型控释化疗系统的基础研究

研究一种新型的控释化疗系统聚-二聚酸一葵二酸一阿霉索(P(DA—SA)-阿霉索(在脑组织内的相容性、体内外释药特性及体外抑瘤特性。将空载体P(DA—SA)分别植入实验动物脑内，观察局部反应；用紫外线光谱测定P(DA—SA)-阿霉素在磷酸盐缓冲液及兔脑内的控释特性；流式细胞仪检测P(DA—SA)-阿霉索诱发的胶质瘤细胞株的凋亡。P(DA—SA)在兔脑内引起的反应较轻，与明胶海绵相比无明显差异。P(DA—SA)-阿霉素在体内外释药速率稳定，控释时间达3周。控释剂组的胶质瘤细胞凋亡率达69．9％，与对照组有明显差异。P(DA—SA)具有良好的组织相容性，P(DA—SA)-阿霉索控释剂在体内外的控释效果理想，杀伤效应明显，该控释化疗系统有很好的临床应用价值。

Basic Study of Biodegradable Controlled Release Chemotherapy

This study sought to assess the biocompatibility of P(DA SA) Adriamycin, a new controlled release chemotherapy system, in rabbit brain, and to examine its controlled release effect both in vitro and in vivo and its curative effects in vitro . The reaction of animal brain to the implanted P(DA SA) or P(DA SA) Adriamycin was observed. The controlled release profiles in phosphate buffer solutions and in rabbit brain were measured by UV spectrometry. Then, through flow cytometer, the rate of apoptosis in cultured glioma cells was tested. The reaction of rabbit brain to P(DA SA) polymer was moderate and not signifiicantly different from that to Gelfoam. The controlled release rate of P(DA SA) Adriamycin in vitro and in vivo was stable and the duration of controlled release of P(DA SA) Adriamycin spanned three weeks. The rate for apoptosis of glioma cells of P(DA SA) Adriamycin group was 69.9%, which was significantly higher than that of the control group.In conclusion, P(DA SA) Adriamycin controlled release chemotherapy system that bears curative effect has favorable controlled release effect and good biocompatibility in rabbit brain. This system has potential value in treatment of malignant brain tumor.