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Comparison of different culture media for isolation ofChlamydia trachomatis by cell culture on HeLa cells |
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| Authors: | P. Herbrink M. Zuyderwijk-Zwinkels B. Niesters J. Wagenvoort W. Van Dijk |
| Affiliation: | (1) Department of Research and Development, Diagnostic Centre SSDZ, PO Box 5010, 2600 GA Delft, The Netherlands;(2) Department of Clinical Microbiology, Diagnostic Centre SSDZ, PO Box 5010, 2600 GA Delft, The Netherlands;(3) Department of Dermatovenerology, University Hospital, Rotterdam, The Netherlands;(4) Department of Clinical Microbiology, University Hospital, Rotterdam, The Netherlands |
| Abstract: | The isolation yield and number ofChlamydia trachomatis inclusions was compared using DEAE-dextran pretreated HeLa cells cultured in four different media: Eagle's minimal essential medium (EMEM) with and without cycloheximide and Dulbecco's modification of EMEM (DMEM) with and without cycloheximide. Using DMEM without cycloheximide the number of inclusions was significantly higher than using EMEM without cycloheximide. In addition, the size of the inclusions was greatly enhanced. Use of DMEM or EMEM with cycloheximide yielded results comparable to those obtained with DMEM without cycloheximide. |
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