腹膜透析相关性腹膜炎兔模型的病理形态学观察

用 12只新西兰大白兔分为腹膜炎组和对照组。腹膜炎组注射含活金黄色葡萄球菌的透析液 ,对照组为含生理盐水的透析液 ;观察腹膜透析相关性腹膜炎兔模型的病理变化。结果发现 :(1)腹膜炎组腹膜间皮细胞增生 ,水肿 ,血管扩张 ,炎症细胞浸润 ,对照组无变化。 (2 )腹膜炎组腹膜透液中葡萄糖浓度除 0min外 ,其他各时间点D/D0 葡萄糖均低于对照组 (P <0 .0 1)。 (3)腹膜炎组腹透液中肌酐浓度与血浆肌酐浓度比值 (D/Dcr)显著高于对照组中的浓度比值 (P <0 .0 5 )。 (4 )腹膜炎组透析液中WBC计数除O点外 ,其余各时间点明显高于对照组 (P <0 .0 5 )。提示新西兰兔腹膜注射含活金黄色葡萄球菌 48h后 ,进行腹膜透析时 ,其腹膜病理改变与临床腹膜透析并发腹膜炎特征基本一致

Effect of peritonitis pathomorphology on rabbit model in peritoneal dialysis

The study about peritonitis rabbit model is rarely, although peritonitis have been considered mainly complicaton in peritoneal dialysis. Twelve New Zealand rabbit were divided into peritonitis group and control group. The peritonitis group rabbit were injected with the dialysate containting alive staphylococcus aureus and the control ones with 0.9% saline into the peritoneum. The pathomorphology changes of the peritonitis were observed. The results showed (1) There was proliferation and swollen of mesothelial cells, vessel dilation and infiltration of inflammation cells in the peritoneum in the peritonitis group; and no such changes in the control group. (2) The D/D 0 glucose concentration in the dialysate of peritonitis group was lower than that of the control ones at any point except the 0 min (P<0.01). (3) The ratio of Cr concentration in the dialysate to Cr concentration in the plasma in the peritnitis group was significantly higher than that of the control group (P<0.05). (4) The WBC count in the dialysate of peritonitis group was obviously higher than that of the control group all points except 0. These results implied that after injection of alive staphylococcus aureus to the peritoneal cavity of New Zealand rabbit for 48 hours, the pathomorphology change was similar with the characteristics of peritonitis complicated with peritoneal dialysis in patient