茵陈术附汤对阴黄证大鼠肝细胞凋亡及Bcl-2和Bax表达的影响

目的　观察阴黄证大鼠肝细胞凋亡和凋亡相关蛋白Bcl 2、Bax的表达以及茵陈术附汤对其的影响。方法　 48只大鼠随机分为 4组 :正常对照组 ,阴黄模型组 ,阳黄对照组 ,茵陈术附汤组。采用TUNEL和免疫组化法检测大鼠肝细胞凋亡及Bcl 2、Bax表达。结果　阴黄证大鼠肝细胞凋亡程度明显高于阳黄及正常对照组 (P <0 .0 1) ,茵陈术附汤组肝组织Bcl 2蛋白表达明显高于阴黄模型组 (P <0 .0 5) ,且其Bax蛋白表达明显低于阴黄模型组 (P <0 .0 5)。结论　茵陈术附汤通过促进Bcl 2表达和抑制Bax表达阻断阴黄证大鼠肝细胞凋亡 ,可能是其治疗阴黄证的机制之一

Hepatocyte apoptosis and the expression of Bcl-2 and Bax in Yin-jaundice rats

ObjectiveTo investigate the effects of Y inchen Shufu Decoction on hepatocyte apoptosis and the expression of its apoptos is-regulating gene Bcl-2 and Bax in Yin-jaundice rats. Mothods STBZForty-eight rats were divided randomly into 4 groups: the normal con trol group, Yin-jaundice model group, Yang-jaundice model group, Yinchen Shufu Decoction treatment group. The TUNEL assay and the immunohistochemistry assay w ere used to detect the apoptosis of hepatocytes and the expression of Bcl-2 and Bax in hepatocytes respectively. ResultsThe rate of a poptosis cells in Yin-jaundice model group was higher significantly than that i n Yang-jaundice model group and normal control group (P<0.01),the express ion of Bcl-2 in Yinchen Shufu Decoction treatment group was higher significant ly than that in Yin-jaundice model group(P<0.01 or P<0.05),and the e xpression of Bax in it was lower significantly than that in Ying-jaundice model group(P<0.01 or P<0.05). ConclusionYinchen Shufu Decoction can prevent hepatocyte apoptosis perhaps by up-regulating the expression of Bcl-2 and down-regulating the expression of Bax. It is one of t he mechanisms of its treatment on Yin-jaundice.