| 10例慢性丙型肝炎病人血清HCV RNA检测及基因分型 |
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| 引用本文： | 张敏,庄辉.10例慢性丙型肝炎病人血清HCV RNA检测及基因分型[J].传染病信息,2000,13(3):114-115. |
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| 作者姓名： | 张敏 庄辉 |
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| 作者单位： | [1]北京解放军第三0二医院 [2]北京医科大学微生物学系 |
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| 摘 要： | 应用Ａｍｐｌｉｃｏｒ ＰＣＲ试剂盒检测１０例慢性丙型肝炎和３例慢性乙型肝炎病人血清ＨＣＶ ＲＮＡ，并对其中３例ＨＣＶ ＲＮＡ阳性者ＩＮＮＯ－ＬｉｐＡ基因分型试剂盒进行分型。结果３例慢性丙型肝炎为ＨＣＶ ＲＮＡ阳性，基因型均为１ｂ型。
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| 关 键 词： | PCR 基因分型 慢性丙型肝炎 HCV－RNA |
Detection and genotype determination of serum HCV RNA in 10 patients with chronic hepatitis C |
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| Zhang Min Zhang Lingxia Zhuang Hui et al Hospital of PLA,Beijing.Detection and genotype determination of serum HCV RNA in 10 patients with chronic hepatitis C[J].Infectious Disease Information,2000,13(3):114-115. |
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| Authors: | Zhang Min Zhang Lingxia Zhuang Hui Hospital of PLA Beijing |
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| Institution: | Zhang Min Zhang Lingxia Zhuang Hui et al 302 Hospital of PLA,Beijing 100039 |
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| Abstract: | Objective To detect the serum HCV RNA in patients with chronic hepatitis and determine its genotypes. Methods The serum HCV RNA was detected in 10 patients with hepatitis C and 3 with hepatitis B using Amplicor PCR box. Then the positive samples were genotyped by INNO-LipA genotyping box. Results Three patients with chronic hepatitis C were HCV RNA positive. Meanwhile, they were all genotyped as lb. Conclusion Amplicor PCR box has high specificity in detecting hepatitis C virus infection. |
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| Keywords: | Viral hepatitis PCR Genotype |
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