喉鳞状细胞癌中PTEN和Caspase-3的表达及临床意义

目的 :研究抑癌基因PTEN和半胱氨酸蛋白酶Caspase 3在喉鳞状细胞癌中的表达及临床意义。 方法 :应用免疫组织化学SP法检测 2 0例正常喉组织、6 0例声带不典型增生、2 89例喉癌组织中PTEN ,Caspase 3的表达。结果 :正常喉组织、声带不典型增生、喉癌组织中PTEN阳性表达率分别为 10 0 .0 %、86 .7%、5 8.8% ,三者之间差异具有统计学意义 (均 P <0 .0 5 ) ;Caspase 3阳性表达率分别为 85 .0 %、6 1.7%、4 8.1% ,三者之间差异具有统计学意义 (均P <0 .0 5 )。另外 ,Caspase 3与PTEN在喉癌中的表达具有正相关性 (P <0 .0 1)。结论 :喉鳞状细胞癌中抑癌基因PTEN ,Caspase 3的表达异常可能与喉癌的发生、发展有关。

Expression and significance of PTEN and Caspase-3 in laryngeal squamous cell carcinoma

Objective:To elucidate the expression and significance of PTEN and Caspase-3 in laryngeal squamous cell carcinoma.Method:Immunochemical method was used to study 289 cases of laryngeal carcinoma,including 60 cases atypical hyperplasia of vocal fold and 20 cases of normal laryngeal tissues.Result:The PTEN gene was expressed in 58.8% laryngeal carcinoma tissues, in 86.7% atypical hyperplasia of vocal fold and 100.0% normal laryngeal tissues. The differences were significance(P<0.05).The Caspase-3 was expressed in 48.1% laryngeal carcinoma tissues, in 61.7% atypical hyperplasia of vocal fold and in 85.0% normal laryngeal tissues. The differences were significance(P<0.05). In addition, there was a positive correlation between the expression of Caspase-3 and PTEN(P<0.01).Conclusion:Expression of PTEN and Caspase-3 in laryngeal carcinoma is different from that of normal laryngeal tissues, which may play an important role in the tumorigenesis and development of laryngeal carcinoma.