| 缺血再灌注对鼠脑血管内皮生长因子表达的影响 |
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| 引用本文： | 程敬君,匡培根. 缺血再灌注对鼠脑血管内皮生长因子表达的影响[J]. 中国神经免疫学和神经病学杂志, 2001, 8(1): 3-4,8 |
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| 作者姓名： | 程敬君 匡培根 |
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| 作者单位： | 1. 军事医学科学院附属医院内二科，北京 100039
2. 解放军总医院神经介质室, 北京 100853 |
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| 基金项目： | 军事医学科学院创新基金资助项目(No.201204) |
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| 摘 要： | 目的 了解血管内皮生长因子（VEGF）在一过性全脑缺血再灌注鼠脑表达的动态变化。方法 采用Pulisnelli改良法四血管堵塞全脑缺血再灌注模型，用免疫组织化学方法观察了全缺血15min再灌注2－14d时，VEGF表达的动肪变化。结果 全脑缺血15min再灌注6h VEGF即可在大脑皮层，纹状体及丘脑等区域的血管内皮细胞表达，1d达高峰，一直持续到缺血后再灌注3d，结论 脑缺血后再灌注可上调VEGF在大脑皮质，纹状体及丘脑等区域内皮细胞的表达，VEGF可能通过促进血管内皮细胞生长对缺血性神经元损伤起一定的作用。
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| 关 键 词： | 脑缺血再灌注 血管内皮生长因子 免疫组织化学 |
| 文章编号： | 1006-2963(2001)01-03-03 |
Expression of VascularEndothelial Growth Factor in Rat Brains Following Transient Global Ischemia |
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| CHENG Jing jun ,KUANG Pei gen. Expression of VascularEndothelial Growth Factor in Rat Brains Following Transient Global Ischemia[J]. Chinese Journal of Neuroimmunology and Neurology, 2001, 8(1): 3-4,8 |
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| Authors: | CHENG Jing jun KUANG Pei gen |
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| Affiliation: | CHENG Jing jun 1,KUANG Pei gen 2 |
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| Abstract: | Objective To investigate the time course of changes of vascular endothelial growth factor (VEGF) expression in rat brains following transient global ischemia.Methods The four vessel occlusion ischemic model of Sprague Dawley rats were made for 15 minutes and recirculated for 2h to 14 days. The expression of VEGF was examined by means of immunohistochemistry method.Results Expressions were rapidly induced (with 6 h) in the cerebral cortex, striatum and thalamus by transient global ischemia (15 min), peaked at 1 day, and lasted at least 3 days.Conclusions These findings suggest that induction of VEGF is up regulated by ischemia. VEGF may play an important role in post ischemia cerebral injury by the maintenance and induction of growth of vascular endothelial cells. |
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| Keywords: | cerebral ischemia vascular endothelial growth factor (VEGF) immunohistochemistry |
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