Evaluation of DNA repair inhibition by antitumor or antibiotic drugs using a chemiluminescence microplate assay |
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| Authors: | Barret, JM Salles, B Provot, C Hill, BT |
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| Affiliation: | Departement de Cancerologie Experimentale, Institut de Recherche Pierre Fabre, Castres, France. |
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| Abstract: | A recently derived in vitro chemiluminescence assay (Salles et al. [1995]Anal. Biochem., 232, 37-42) has been used to investigate the effects of apanel of twenty-two anticancer drugs and certain antibiotics on theexcision repair activity of cell-free extracts from the human cell line,HeLa. This methodology, termed the 3D (Damaged DNA Detection) assay, basedon the in vitro excision repair assay previously developed (Wood et al.[1988] Cell, 53, 97-106) has provided data indicating definite in vitroinhibition of DNA repair by actinomycin D, aphidicolin, doxorubicin,distamycin A and mithramycin A. This assay therefore offers the potentialfor identifying agents with the ability to inhibit DNA repair. |
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