SiRNA对大鼠钙调神经磷酸酶活性及VSMCs增殖的影响

目的:观察神经肽Y(NPY)刺激的大鼠血管平滑肌细胞(VSMCs)增殖中钙调神经磷酸酶(CaN)活性和c-fos表达水平的变化,以及RNA干扰抑制CaN活性的作用.方法:采用组织贴块法体外原代培养大鼠胸主动脉平滑肌细胞;NPY刺激培养的大鼠VSMCs增殖;RNA干扰特异性抑制CaN的活性及其mRNA的表达,阻断NPY刺激的大鼠VSMCs中CaN依赖的信号转导通路,观察对CaN活性、细胞增殖、核内原癌基因c-fos表达水平的变化.结果:NPY可增加VSMCs的CaN活性,加快细胞增殖及增加核内c-fos表达水平.RNA干扰抑制CaN活性后,明显降低NPY刺激的VSMCs增殖及核内c-fos表达水平.结论:CaN参与了NPY刺激的VSMCs增殖,RNA干扰通过抑制CaN的活性可阻断NPY诱导的VSMCs增殖作用.

Inhibitory effect of siRNA on calcineurin activity and vascular smooth muscle cell proliferation in rats

AIM: To study the changes of calcineurin(CaN) activity and c-fos expression level in the proliferation of rat vascular smooth muscle cells(VSMCs) stimulated by neuropeptide Y(NPY),and the inhibition of siRNA on the activity of CaN and the proliferation of VSMCs.METHODS: VSMCs from thoracic aorta in rats were cultured in vitro using tissuepiece anchorage primary culture.VSMCs were treated with NPY and the cell proliferation was observed.RNA interference(RNAi) was used to block CaN-depended signal transduction pathway in rat VSMCs stimulated by NPY,then the activity of CaN,the proliferation of VSMCs and the expression level of c-fos were tested.RESULTS: NPY could increase the CaN activity,the proliferation of VSMCs,and the expression level of c-fos.RNAi inhibited the activity of CaN,VSMCs proliferation and c-fos expression in VSMCs.CONCLUSION: CaN takes part in the proliferation of cultured VSMCs stimulated by NPY,while RNAi blocks the proliferation of VSMCs stimulated by NPY by inhibiting the activity of CaN.