不同配伍柴郁温胆汤对抑郁模型大鼠海马CREB和BDNF表达的影响

目的观察柴郁温胆汤及其不同配伍组合对抑郁模型大鼠海马环磷酸腺苷反应元件结合蛋白(CREB)和脑源性神经营养因子(BDNF)表达的影响。方法健康雄性SD大鼠70只,随机分为正常组、模型组、复方组、化痰组、调气血组、养心脾组和马普替林组。除正常组外,各组采用孤养加慢性轻度不可预见性刺激方法建立大鼠抑郁模型,从应激第2天起分别灌胃给药至实验第23天。采用免疫组化法检测大鼠海马CA3区CREB和BDNF的表达。结果与正常组相比,模型组大鼠海马CREB和BDNF表达的总面积、阳性细胞数和平均光密度均明显降低(P<0.01)。与模型组相比,复方组、化痰组、马普替林组能够显著上调大鼠海马CREB和BDNF表达(P<0.05或P<0.01);养心脾组能够提升CREB表达的总面积和阳性细胞数(P<0.05);调气血组能够提升BDNF表达的平均光密度(P<0.05)。结论柴郁温胆汤能够明显上调大鼠海马CREB和BDNF表达,其中以化痰药的配伍组合作用较明显。

Effect of the Chaiyu Wendan Decoction of Different Compatibilities on Expressions of CREB and BDNF in Hippocampus of Rat Models with Depression

Objective To explore the effect of Chaiyu Wendan Decoction Decoction containing Chaihu(Radix bupleuri and Yujin(Radix curcumae) for gallbladder diseases] and its different compatibilities on the expressions of cyclic adenosine monophosphate(cAMP) response element binding protein(CREB) and brain-derived neurotrophic factor(BDNF) in the hippocampus of rat models with depression.Methods Seventy healthy male SD rats were randomized into normal group,model group,Chaiyu Wendan group,dissolving phlegm group,regul...