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| 白附子对正常和肾阳虚证大鼠毒性作用的差异 | |
| 引用本文： | 谭勇,李健,陈杲,吕诚,赵宏艳,吕爱平.白附子对正常和肾阳虚证大鼠毒性作用的差异[J].中医杂志,2011,52(14):1229-1232. |
| 作者姓名： | 谭勇 李健 陈杲 吕诚 赵宏艳 吕爱平 |
| 作者单位： | 1. 中国中医科学院中医临床基础医学研究所,北京市东直门内南小街16号,100700 2. 北京中医药大学基础医学院 3. 中国中医科学院中医基础理论研究所 |
| 基金项目： | 国家“十一五”科技支撑计划重点课题，北京市教育委员会科技研究面上项目 |
| 摘 要： | 目的从代谢组学角度探讨正常和肾阳虚证大鼠对白附子的毒性反应的差异。方法将80只SD雄性大鼠随机分为肾阳虚证模型组和正常组,采用肌肉注射氢化可的松琥珀酸钠的方法造模,造模结束后,把两组大鼠随机分为低剂量、小剂量、中剂量和大剂量给药组和空白组,每组8只;对各给药组大鼠连续14d灌胃给予不同浓度的白附子溶液,剂量依次为0.32、0.64、1.28、2.56g/(kg·d),空白组给予生理盐水;末次给药24h后取血浆,采用高效液相色谱-质谱联用的代谢组学技术检测血清促肾上腺皮质激素(ACTH)、皮质醇(COR)、环磷酸腺苷(cAMP)和环磷酸鸟苷(cGMP)。结果氢化可的松造模可引起血浆代谢物溶血磷脂酰乙醇胺(LPE)水平和溶血磷脂酰胆碱(LPC)水平的下调。正常大鼠和肾阳虚证大鼠给予白附子后两者内源性代谢产物存在差异,随着白附子剂量的增加,正常大鼠LPC水平和LPE水平有下降的趋势,而模型大鼠LPC和LPE水平基本保持在稳定状态。结论肾阳虚证大鼠对白附子的毒性反应低于正常大鼠,印证了在证候理论指导下进行中药毒性评价的合理性。 |
| 关 键 词： | 白附子 肾阳虚证 毒性反应 代谢组学 |
Difference of Toxic Effect of Rhizoma Typhonii on Normal and Kidney-yang Insufficiency Rats |
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| TAN Yong,LI Jian,CHEN Gao,Lü Cheng,ZHAO Hongyan,Lü Aiping.Difference of Toxic Effect of Rhizoma Typhonii on Normal and Kidney-yang Insufficiency Rats[J].Journal of Traditional Chinese Medicine,2011,52(14):1229-1232. | |
| Authors: | TAN Yong LI Jian CHEN Gao Lü Cheng ZHAO Hongyan Lü Aiping |
| Institution: | TAN Yong1,LI Jian2,CHEN Gao1,Lü Cheng1,ZHAO Hongyan3,Lü Aiping1(1.Institute of Basic Research in Clinical Medicine,China Academy of Chinese Medical Sciences,Beijing 100700,2.School of Basic Medical Sciences,Beijing University of Chinese Medicine,3.Institute of Basic Theory of TCM,China Academy of Chinese Medical Sciences) |
| Abstract: | Objective To explore the difference of toxic effect of Baifuzi (Rhizome Typhonii) on normal and kidney-yang insufficiency rats from the viewpoint of metabonomics.Methods Eighty SD male rats were randomized into normal group and kidney yang deficiency model group.After making the kidney yang deficiencyt model by injecting intramuscularly hydrocortisoni natrii succinas,the rats of two groups were randomized into the low,small,medium,large dose sub-groups and blank group with 8 in each.The medicated rats were ... |
| Keywords: | Rhizome Typhonii kidney-yang insufficiency syndrome toxic reaction metabonomics |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |