中老年人群小肠脂肪酸结合蛋白FABP2基因54位密码子多态性与血脂水平的关系

背景：有研究表明，在不同人群中，突变型54TFABP2与血脂障碍以及代谢综合征的其他特征相关。 目的：调查中老年人群小肠脂肪酸结合蛋白FABP2基因多态性频率分布，分析突变型54TFABP2基因与血脂水平的关系。 设计：病例一对照分析。 单位：河北北方学院生物化学教研室和解放军第二五一医院检验科。 对象：选择2003—10/2005-04在解放军第二五一医院体检中心进行体检的中老年人469名，男217名，平均年龄（56±10）岁；女252名，平均年龄（55±13）岁。除外肝、肾功能异常者，相互间无血缘关系；患者均对本实验均知情同意。实验已经医院伦理委员会批准。 方法：①空腹12h后，采用全自动生化仪（Olympus AU6400）测定血浆总胆固醇、三酰甘油、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、载脂蛋白AⅠ、载脂蛋白B水平。②采集空腹静脉血1mL，枸橼酸钠抗凝，分离白细胞，蛋白酶K消化，酚，氯仿抽提基因组DNA。采用PCR-限制性片段长度多态性技术检测各组基因型分布频率。 主要观察指标：①血脂水平。②FABP254位点基因型分布频率。 结果：①频率分布：男性54A／TFABP2基因型频率分布为A／A0．48，A／T0．42，T／T0．10：女性为A／A0．44，A／T0．46，T／T0．10。男性和女性突变型54T等位基因频率分别为0．31，0．33。男女间频率分布情况比较，差异无显著性意义（P〉0．05）。②血脂：男性，54T等位基因携带者血浆低密度脂蛋白胆固醇、载脂蛋白B高于54A等位基因携带者，差异有显著性意义（P〈0．05）；女性，54T等位基因携带者血浆总胆固醇、低密度脂蛋白胆固醇高于54A等位基因携带者，差异有显著性意义（P〈0．05）。 结论：在中老年人群中，FABP2基因多态性频率分布与性别无关；54TFABP2基因携带者有高血脂特征。

Association between codon 54 polymorphism of intestinal fatty acid-binding protein 2 gene and plasma lipids in middle-aged and old populations

BACKGROUND： Studies have shown that alanine （A） to threonine （T） substitution at codon 54 of intestinal fatty acid-binding protein （FABP2） in different populations is associated with dyslipidemia and other characteristics of metabolic syndrome.
OBJECTIVE： To investigate the frequency of encoding 54Ala/Thr （A/T） single nucleotide polymorphism in the FABP2 in middle-aged and old people, and explore the association between 54T FABP2 and plasma lipids.
DESIGN： A case-controlled analysis.
SETTING： Department of Biochemistry, Hebei North University and Department of Clinical Laboratory, the 251 Hospital of Chinese PLA.
PARTICIPANTS： 469 physical examinees were selected from the Medical Examination Center, the 251 Hospital of Chinese PLA between October 2003 and April 2005. The subjects included 217 males with mean age of （56± 10） years, and 252 females with mean age of （55 ± 13） years. Only people with normal liver and kidney function, and with no blood relation were recruited. The informed consent to this study was obtained from all subjects. The experiment was admitted by Hospital Ethics Committee.
METHODS： ①After fasting for 12 hours, automatic analyzer （Olympus AU 6400） was adopted to measure plasma total cholesterol （TC）, triglyceride （TG）, high density lipoprotein cholesterol （HDL-C）, low density lipoprotein cholesterol （LDL-C）, apoprotein A1（Apo A1） and Apo B levels. ②1 mL venous blood was extracted and immediately mixed with anti-coagulants containing citric acid, natrium citricum and glucose. White blood cells were separated and genomic DNA was isolated using standard methods with proteinase K digestion and phenol/chloroform purification. The genotype distribution frequency in each group was detected by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）.
MAIN OUTCOME MEASURES： ①Plasma TC, TG, HDL-C, LDL-C, Apo Aland Apo B levels; ②Distributions of FABP2 genotypes at codon 54. RESULTS： ①The gen