芍石护睛胶囊醇沉工艺的优选

目的:优选芍石护睛胶囊的醇沉最佳工艺条件。方法:HPLC法测定醇沉液中芍药苷的含量;采用正交试验法考察醇沉前水煎液相对密度、醇沉浓度、醇沉时间3因素对醇沉工艺有效成分含量的影响。结果:醇沉前水煎液相对密度对醇沉工艺有显著影响(P<0.05)。芍石护睛胶囊醇沉工艺最佳条件即煎煮液浓缩密度为1.10～1.15,加入乙醇使含醇量为70%,醇沉时间为12h。结论:该工艺缩短了生产周期,降低了生产成本,适用于实际生产。

Optimizing of Ethanol Precipitation Technology of Shaoshi Hujing Capsules by Orthogonal Design

OBJECTIVE:To optimize the ethanol precipitation condition for Shaoshi hujing capsules.METHODS:The content of Paeoniflorin in ethanol precipitated solution was determined by HPLC.Effects of three factors-relative density of water decoction before decoction,concentration of ethanol and time of reaction on the content of paeoniflorin in the ethanol precipitation were investigated by orthogonal design.RESULTS:The relative density of water decoction before decoction had a significant impact on ethanol precipitation(P<0.05).The optimum ethanol precipitation condition of Shaoshi hujing capsules was proved to be as follows:the concentrated density of the decoction solution was 1.10～ 1.15;the alcohol content of ethanol was 70%,and the ethanol precipitation time was 12h.CONCLUSON:The production cycle was shortened and the production cost was lowered by this optimum technology,which thus applicable for the practical production of Shaoshi hujing capsules.