Preclinical diagnosis of African swine fever in contact-exposed swine by a real-time PCR assay |
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| Authors: | Zsak L Borca M V Risatti G R Zsak A French R A Lu Z Kutish G F Neilan J G Callahan J D Nelson W M Rock D L |
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| Affiliation: | Plum Island Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 848, Greenport, NY 11944-0848, USA. lzsak@piadc.ars.usda.gov |
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| Abstract: | A fluorogenic probe hydrolysis (TaqMan) PCR assay for African swine fever virus (ASFV) was developed and evaluated in experimentally infected swine. This sensitive and specific one-step single-tube assay, which can be performed in 2 h or less, detected viral DNA in tonsil scraping samples 2 to 4 days prior to onset of clinical disease. Thus, the assay would have application for preclinical diagnosis of African swine fever and surveillance and/or emergency management of a disease outbreak. |
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