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| Effect of lipopolysaccharide on diarrhea and gastrointestinal transit in mice： Roles of nitric oxide and prostaglandin E2 | |
| 引用本文： | Liang YC,Liu HJ,Chen SH,Chen CC,Chou LS,Tsai LH. Effect of lipopolysaccharide on diarrhea and gastrointestinal transit in mice： Roles of nitric oxide and prostaglandin E2[J]. World journal of gastroenterology : WJG, 2005, 11(3): 357-361. DOI: 10.3748/wjg.v11.i3.357 |
| 作者姓名： | Liang YC Liu HJ Chen SH Chen CC Chou LS Tsai LH |
| 作者单位： | Graduate Institute of Biomedical Technology,Department of Physiology,Department of Internal Medicine,Department of Internal Medicine,Department of Physiology,Department of Physiology Taipei Medical University,Taipei,Taiwan,China,College of Medicine,Taipei Medical University,Taipei,Taiwan,China,Taipei Medical University Hospital,Taipei Medical University,Taipei,Taiwan,China,Min-Sheng Healthcare,Taoyuan,Taiwan,China,College of Medicine,Taipei Medical University,Taipei,Taiwan,China,College of Medicine,Taipei Medical University,Taipei,Taiwan,China |
| 基金项目： | Supported by the National Science Council of Taiwan (NSC 92-2320-B-038-027) the Min-Sheng Healthcare (93MSH-TMU-006) |
| 摘 要： | AIM: To investigate the effect of lipopolysaccharide (LPS) on the diarrheogenic activity, gastrointestinal transit (GIT), and intestinal fluid content and the possible role of nitric oxide (NO) and prostaglandin E2 (PGE2) in gastrointestinal functions of endotoxin-treated mice. METHODS: Diarrheogic activity, GIT, and intestinal fluid content as well as nitric oxide and PGE2 products were measured after intraperitoneal administration of LPS in mice. RESULTS: LPS dose-dependently accumulated abundant fluid into the small intestine, induced diarrhea, but decreased the GIT. Both nitric oxide and PGE2 were found to increase in LPS-treated mice. Western blot analysis indicated that LPS significantly induced the protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 in mice intestines. Pretreatment with NG-nitro-L-arginine-methyl ester (L-NAME, a non-selective NOS inhibitor) or indomethacin (an inhibitor of prostaglandin synthesis) significantly attenuated the effects of LPS on the diarrheogenic activity and intestine content, but reversed the GIT. CONCLUSION: The present study suggests that the pathogenesis of LPS treatment may mediate the stimulatory effect of LPS on nitric oxide and PGE2 production and NO/ prostaglandin pathway may play an important role on gastrointestinal function. |
| 关 键 词： | 脂多糖 痢疾 胃肠疾病 老鼠 含氮氧化物 前列腺素E2 腹泻 |
| 收稿时间： | 2004-05-27 |
Effect of lipopolysaccharide on diarrhea and gastrointestinal transit in mice: roles of nitric oxide and prostaglandin E2 |
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| Liang Yu-Chih,Liu Hung-Jung,Chen Sheng-Hsuan,Chen Chun-Chin,Chou Liang-Shung,Tsai Li Hsueh. Effect of lipopolysaccharide on diarrhea and gastrointestinal transit in mice: roles of nitric oxide and prostaglandin E2[J]. World journal of gastroenterology : WJG, 2005, 11(3): 357-361. DOI: 10.3748/wjg.v11.i3.357 | |
| Authors: | Liang Yu-Chih Liu Hung-Jung Chen Sheng-Hsuan Chen Chun-Chin Chou Liang-Shung Tsai Li Hsueh |
| Affiliation: | 1. Graduate Institute of Biomedical Technology, Taipei Medical University, Taipei, Taiwan, China 2. Department of Physiology, College of Medicine, Taipei Medical University, Taipei,Taiwan, China 3. Department of Internal Medicine, Taipei Medical University Hospital, Taipei Medical University, Taipei, Taiwan, China 4. Department of Internal Medicine, Min-Sheng Healthcare, Taoyuan, Taiwan, China |
| Abstract: | AIM: To investigate the effect of lipopolysaccharide (LPS) on the diarrheogenic activity, gastrointestinal transit (GIT), and intestinal fluid content and the possible role of nitric oxide (NO) and prostaglandin E2 (PGE2) in gastrointestinal functions of endotoxin-treated mice. METHODS: Diarrheogic activity, GIT, and intestinal fluid content as well as nitric oxide and PGE2 products were measured after intraperitoneal administration of LPS in mice. RESULTS: LPS dose-dependently accumulated abundant fluid into the small intestine, induced diarrhea, but decreased the GIT. Both nitric oxide and PGE2 were found to increase in LPS-treated mice. Western blot analysis indicated that LPS significantly induced the protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 in mice intestines. Pretreatment with NG-nitro-L-arginine-methyl ester (L-NAME, a non-selective NOS inhibitor) or indomethacin (an inhibitor of prostaglandin synthesis) significantly attenuated the effects of LPS on the diarrheogenic activity and intestine content, but reversed the GIT. CONCLUSION: The present study suggests that the pathogenesis of LPS treatment may mediate the stimulatory effect of LPS on nitric oxide and PGE2 production and NO/ prostaglandin pathway may play an important role on gastrointestinal function. |
| Keywords: | Diarrhea Gastrointestinal Transit Ljpopolysaccharide Nitric Oxide Prostaglandin E2 |
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