| 脂氧合酶抑制剂NDGA对乳腺癌细胞 MCF-7生物学特性的影响 |
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| 引用本文： | 刘焕涛,孙靖中,马榕,唐鲁兵.脂氧合酶抑制剂NDGA对乳腺癌细胞 MCF-7生物学特性的影响[J].山东大学学报(医学版),2005,43(10):956-959. |
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| 作者姓名： | 刘焕涛 孙靖中 马榕 唐鲁兵 |
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| 作者单位： | 山东大学齐鲁医院乳腺外科,山东,济南,250012;山东大学齐鲁医院乳腺外科,山东,济南,250012;山东大学齐鲁医院乳腺外科,山东,济南,250012;山东大学齐鲁医院乳腺外科,山东,济南,250012 |
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| 摘 要： | 目的：探讨去甲二氢愈创木酸（nordihydroguaiaretic acid，NDGA）对乳腺癌细胞MCF-7增殖和凋亡的生物学特性的影响。方法：MTT法绘制细胞生长曲线及检测细胞存活率、倒置相差显微镜和扫描电镜观察细胞凋亡、流式细胞仪检测细胞周期和凋亡率。结果：NDGA对乳腺癌细胞MCF-7生长增殖的抑制作用呈剂量和时间依赖效应，阻滞细胞周期于S期，诱导细胞凋亡形成，使细胞表面超微结构发生改变。结论：NDGA能够抑制乳腺癌MCF-7细胞增殖，诱导细胞凋亡。
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| 关 键 词： | 乳腺肿瘤 脂氧合酶 细胞凋亡 侵袭 肿瘤 |
| 文章编号： | 1671-7554（2005）10-0956-04 |
| 收稿时间： | 2004-11-15 |
| 修稿时间： | 2004年11月15 |
Effects of nordihydroguaiaretic acid on breast cancer MCF-7 cells |
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| LIU Huan-tao,SUN Jing-zhong,MA Rong,TANG Lu-bing.Effects of nordihydroguaiaretic acid on breast cancer MCF-7 cells[J].Journal of Shandong University:Health Sciences,2005,43(10):956-959. |
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| Authors: | LIU Huan-tao SUN Jing-zhong MA Rong TANG Lu-bing |
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| Institution: | Department of Breast Surgery, Qilu Hospital of Shandong University,Jinan 250012, Shandong,China |
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| Abstract: | Objective: To explore the effects of nordihydroguaiaretic acid (NDGA) on apoptosis and proliferation of breast cancer MCF-7 cells. Methods: The cells growth curve and survival rate were observed by means of MTT asssy. Morphological variation was investigated by invert phase-contrast microscopy and scanning electron microscopy. Cell cycle analysis and apoptosis rate were evaluated by flow cytometry. Results: NDGA at various concentrations inhibited the growth of MCF-7 cells in a time-and dose-dependent manner, blocked the cell cycle in S phase, induced cells apoptosis and changed the morphology of MCF-7 cells. Conclusion: NDGA can inhibit the proliferation and induce the apoptosis of MCF-7 cells. |
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| Keywords: | Breast neoplasms Lipoxygenase Apoptosis Invasiveness neoplasm |
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