外淋巴瘘的毛细胞凋亡与听力损伤

目的：为探讨外淋巴瘘时的毛细胞凋亡现象及其在听力损伤中的作用。方法：对２５只豚鼠行外淋巴瘘造模后利用光镜，ＴＵＮＥＬ标记技术，耳蜗电图及脑干诱发电位等检测指标。结果：（１）外淋巴瘘早期（０ｈ组，２ｈ组）８只豚鼠形态正常，无毛细胞凋亡，随着外淋巴瘘时程延长，毛细胞凋亡数增多，耳蜗毛细胞凋亡出现概率分别为造瘘后１ｄ组（１／６只），２ｄ组６７％（４／６只）和７ｄ组８０％（４／５只）；（２）外淋巴瘘造模后

Hair cell apoptosis and hearing loss of perilymphatic fistula

To investigate the effect of hair cell apoptosis on hearing loss of perilymphatic fistula(PLF) in guinea pig. Method: Twenty-five guinea pigs with light microscope and TdT mediated biotin dUTPnick-end labelling (TUNEL) techniques. The ECochG and ABR were measured and the data analyzed withstatistics. Result:The apoptosis of hair cell was not revealed in 0-hour-group and 2-hour-group of PLF. Following the time of PLF was longer, the apoptosis of hair cell was more increased. The apoptosis of hair cell wasdemonsted in 1-day-group (1 case, 17 % ), 2-day-group (4 case, 67 % ) and 7-day-group (4 case, 80% ); The amplication of AP was reduced after operation induced PLF. The CAP of experimental ear was significantly higherthan that of control ear (P <0. 01 ) in 1-day-group, 2-day-group and 7-day-group. Conclusion: Apoptosis ofhair cell was appeared in PLF; The apoptosis of hair cell may be one of morphological evidence in hearing lossof PLF.