| Percoll优选对形态正常精子百分率和顶体酶活性的影响 |
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| 引用本文： | 沙艳伟,王洪亮,刘睿智,许宗革,高久春,王忠山.Percoll优选对形态正常精子百分率和顶体酶活性的影响[J].中华男科学杂志,2004,10(4):263-264,268. |
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| 作者姓名： | 沙艳伟 王洪亮 刘睿智 许宗革 高久春 王忠山 |
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| 作者单位： | 1. 吉林大学基础医学院细胞生物学教研室,吉林,长春,130021
2. 吉林大学第一临床医院泌尿外科,吉林,长春,130021 |
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| 摘 要： | 目的 :了解形态正常精子百分率和顶体酶活性在Percoll优选前后的变化。 方法 :30例不育男性精液标本 ,应用计算机精液分析仪进行精子形态分析 ,分光光度比色法检测顶体酶活性。 结果 :Percoll优选后形态正常精子百分率和精子顶体酶活性均明显高于Percoll优选前 (P均 <0 .0 0 1)。 结论 :Percoll法优选精子后形态正常精子百分率和顶体酶活性增加。
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| 关 键 词： | 男性不育 精子形态 顶体酶 Percoll优选法 |
| 文章编号： | 1009-3591(2004)04-0263-03 |
| 修稿时间： | 2003年9月13日 |
Effect of Percoll Selection Technique on Normal Morphology Rate and Acrosin Activity of Human Spermatozoa |
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| Sha Yanwei,Wang Hongliang,Liu Ruizhi,Xu Zongge,Gao Jiuchun,Wang Zhongshan.Effect of Percoll Selection Technique on Normal Morphology Rate and Acrosin Activity of Human Spermatozoa[J].National Journal of Andrology,2004,10(4):263-264,268. |
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| Authors: | Sha Yanwei Wang Hongliang Liu Ruizhi Xu Zongge Gao Jiuchun Wang Zhongshan |
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| Institution: | Department of Cell Biology, School of Basic Medical Sciences, Jilin University, Changchun, Jilin 130021, China. |
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| Abstract: | Objective: To study the effect of Percoll selection technique on normal morphology and acrosin activity of human spermatoza. Methods: The sperm morphology and sperm acrosin activity were analyzed by automated sperm morphology analyzer(ASMA) and spectrocolorimetry. Results: The normal morphology sperm rate and acrosin activity were significantly increased after Percoll selection technique (P< 0.001 ). Conclusion: Percoll selection technique could affect normal morphology sperm ratio and acrosin activity. |
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| Keywords: | male infertility sperm morphology acrosin activity Percoll selection technique |
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