双极电凝镊两种灭菌效果的比较

目的]研究高压蒸汽灭菌法及过氧乙酸熏蒸灭菌法灭菌双极电凝镊的效果。方法]取20件同型号纸塑包装厂家已环氧乙烷灭菌的崭新威力牌双极电凝镊,于手术使用后分别采用高压蒸汽灭菌法和过氧乙酸熏蒸灭菌法处理,比较两种方法的灭菌效果。结果]手术使用后采用高压蒸汽灭菌法,双极电凝镊的带菌量由使用前的390cfu/cm2降为无菌生长,细菌清除率100%。采用过氧乙酸熏蒸灭菌法则视过氧乙酸密闭、开启放置时间而异,新开启的过氧乙酸液用于熏蒸灭菌,双极电凝镊的带菌量由使用前410cfu/cm2降为无菌生长,细菌清除率100%,采用开启低温放置12d的过氧乙酸液熏蒸灭菌双极电凝镊的带菌量由使用前380cfu/cm2降为25cfu/cm2,细菌清除率93.2%,且过氧乙酸熏蒸双极电凝镊腐蚀率达33.3%。结论]高压蒸汽灭菌双极电凝镊具有效果可靠、经济安全、简便无腐蚀的优点。过氧乙酸熏蒸灭菌因溶液稳定性差,灭菌效果有时不可靠,且腐蚀性强,因此尽量不作为双极电凝镊的灭菌法,高压蒸汽灭菌法优于过氧乙酸熏蒸灭菌法。

Comparison on effects of two different ways of sterilization on bipolar coagulation forceps

Objective:To compare the effects of high pressure steam sterilization and peracetic acid fumigating sterilization for disinfection of bipolar coagulation forceps.Methods:Totally 20 pieces of same-type and new Weili brand of bipolar coagulation forceps with paper-plastics package were sterilized with ethylene oxide by factory were taken.And these bipolar coagulation forceps were sterilized with high pressure steam sterilization and peracetic acid fumigating sterilization respectively after operation.Then the sterilization effects of both methods were compared with each other.Results:The germ-carrying amount of bipolar coagulation forceps sterilized with high pressure steam sterilization after operation decreased from 390 cfu/cm 2 before using to zero growth of germ.And the bacteria clearance was 100%.The effects of peracetic acid fumigating sterilization were different based on different closing-opening times of sterilization fluids.Newly-opened peracetic acid fluid used fumigating sterilization made the germ-carrying amount of bipolar coagulation forceps descend from 410 cfu/cm 2 to zero growth.And the bacteria clearance was 100%.However,peracetic acid fluid placing for 12 days after opening used for fumigating sterilization made the germ-carrying amount of bipolar coagulation forceps s descend from 380 cfu/cm 2 before operation usage to 25 cfu/cm 2 after operation.And the bacteria clearance was 93.2%.Moreover,the corrosion rate of bipolar coagulation forceps after peracetic acid fumigating sterilization was 33.3%.Conclusion:High pressure steam sterilization for bipolar coagulation forceps has advantages including reliable effect,economic,safe,simple and no corrosion.Peracetic acid fumigating sterilization solution with poor stability and sterilizing effect was un-reliable sometimes and causticity was powerful,therefore,which was unsuitable as sterilization for bipolar coagulation forceps.The effect of high pressure steam sterilization is superior to peracetic acid fumigating sterilization.