中国人类免疫缺陷病毒（HIV—1）D亚型毒株gag,env和t …

目的 通过对ＨＩＶ－１毒株ｇａｇ，ｔａｔ，ｅｎｖ基因的序列分析，阐明Ｄ亚型ＨＩＶ－１毒株已在中国出现。方法 从１名四川非洲回国营务人员ＨＩＶ感染者（ＳＣ９ ７１２）淋巴细胞（ＰＢＭＣ）中提取前病毒ＤＮＡ，使用套式ＰＣＲ方法分别扩增ＨＩＶ－１的ｇａｇ基因区，ｅｎｖ基因的Ｃ２Ｖ３区和ｔａｔ基因的第一外显子。结果 发现ＳＣ９ ７１２在ｇａｇ区，ｅｎｖ区和ｔａｔ区与国际标准Ｄ亚型毒株的基因距离最近，其中在

Identification and characterization of HIV1 subtype D strain in China

Objective Through sequence analysis of HIV1 gag, tat and env regions , the subtype D HIV1 had been identified appering in China. Methods The blood was collected from HIV1 infected individual (SC9 712) in Sichuan Province. The peripheral blod mononuclear cells (PBMCs) were isolated from the blood by ficoll and the genomic DNA was extracted. The gag gene, the C2V3 of the env gene and the first exon of tat gene were amplified by nestedPCR and sequenced directly. Results The genetic distance respectively is 7.56% in tat gene between SC9 712 and UG274A, 9.59% and 9.94% in gag gene between SC9 712 and K31 and VI203, 13.86% in env gene between SC9 712 and MAL. On the phylogenetic tree the SC9 712 HIV1 strain clustered with the Africa subtype D strains UG274A, K31, VI203 and MAL. The above data demonstrated that SC9 712 was subtype D HIV1 strain. Epidemiological data showed that SC9 712 virus was from a person who had worked in Africa. Conclusion The results indicated that the person who came back from Africa had transmitted the subtype D of HIV1 from Africa to China.