影像学和组织学在评价组织工程骨修复骨缺损中的联合应用

目的 应用影像学和组织学方法联合追踪观察组织工程骨成骨及修复骨缺损的过程,并加以分析评价. 方法抽取兔自体骨髓基质干细胞于体外培养增殖,并与煅烧骨复合培养1周后,将其植入12 mm长桡骨缺损区,于术后4、8、12周采用放射线、CT断层扫描、三维重建进行形态学观察,之后取材行组织学检查. 结果组织工程骨植入术后随时间延长逐渐成骨并修复骨缺损,术后12周时,放射线和CT见骨折线完全消失,组织工程骨密度与正常桡骨相近,外形平整、曲线光滑,与正常桡骨完全融为一体;组织学上见成熟骨小梁规则排列,骨结构成熟,板层状皮质骨形成,支架组织松散,呈部分降解状. 结论联合应用放射线、CT断层扫描、三维重建以及组织学检查是全程、系统、细致地观察组织工程骨成骨及其修复骨缺损过程科学、可靠的方法.

Imageology and histology to characterize tissue engineering repair of bone defects in rabbits

Objective To evaluate the effects of imageology and histology in characterizing tissue engineering repair of bone defects in rabbits. Methods The autologous bone marrow stroma cells (BMSCs) were obtained from the rabbits and cultured in vitro. After proliferation, the cells were incubated together with true bone ceramic (TBC) for 1 week, which were then imbedded into the 12 mm defects to repair the radius. X-ray and computer tomography (CT) exams and three-dimensional reconstruction were performed at 4, 8 and 12 weeks postoperatively for morphological observation before histological examination of the cells. Results After engineering repair, bone formation completed gradually and the defects of the bone were repaired. At 12 weeks postoperatively, X-ray and CT showed that the fracture lines disappeared completely, and the density of the engineering bone was similar to that of the normal radius. The smooth repaired bone grew into the normal radius. Histological examination showed regular arrangement of the mature bone trabecula and architecture, formation of the lamellar cortical bone. and partial degradation of the framework. Conclusion Combined application of radiography, CT, three-dimensional reconstruction and histology is a scientific and reliable method for characterization of tissue engineering repair of bone defects.