大豆异黄酮对牙周炎大鼠牙槽骨吸收影响

目的 研究补充大豆异黄酮对骨质疏松实验型牙周炎大鼠牙槽骨吸收的影响。方法 50只雌性SD大鼠随机分为5组:假手术组、去卵巢对照组、雌激素对照组和大豆异黄酮低、高剂量组。各组动物术后28 d结扎上颌第一磨牙牙颈部诱导牙周炎,第65 d测量上颌和第一磨牙丧失值(periodonal bone loss,PBL),比较牙周支持率(peri-odontal bone support,PBS),检测血清中骨代谢生化指标总碱性磷酸酶(total alkaline phosphatase,AKP)、抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphatase,TRAP)、骨钙素(bone gla protein,BGP)。结果 大豆异黄酮组与去卵巢对照组比较,PBL、PBS、AKP、TRAP和BGP指标的差异有统计学意义(P<0.05),而与假手术对照和雌激素对照组比较差异无统计学意义(P>0.05)。结论 雌激素缺乏是促进实验性牙周炎大鼠牙槽骨吸收的重要原因之一,补充大豆异黄酮可有效预防因此产生的牙槽骨高度降低,降低失牙风险。

Effect of soybean isoflavone on alveolar bone absorption in osteoporosis rats with experimental periodontitis

Objective To evaluate the effects of soybean isoflavone supplementation on alveolar bone absorption in osteoporosis rats with experimental periodontitis.Methods Fifty female SD rats were randomly divided into 5 groups,including sham group,Ovx group.Twenty-eight days later,the upper first molars were placed ligature to induce periodontitis.On the 65th day,the periodontal bone loss(PBL) and periodontal bone support(PBS) were measured,and the serum bone metabolism index,such as total alkaline phosphatase(AKP),tartrate-resistant acid phosphatase(TRAP) and bone gla protein(BGP) were also determined.Results PBL,PBS,AKP,TRAP and BGP in soybean isoflavone groups showed significant difference with the Ovx group(P<0.05),but no difference with sham group and estrogen control group(P>0.05).Conclusion Estrogen deficiency is one of the factors that cause alveolar bone absorption in rats with experimental periodontitis,and supplementation of soybean isoflavone can effectively prevent the decrease of alveolar bone height and teeth loss.