注射用胡黄连总苷对ConA引起小鼠急性免疫性肝损伤的保护作用研究

目的利用Concanavalin A（ConA）引起的免疫性肝损伤模型，对注射用胡黄连总苷的保肝活性和量效关系进行研究。方法注射用胡黄连总苷静脉给予ICR小鼠（0．5—8mg·kg-1×5），于末次给药后2h，动物尾静脉ConA20mg·kg-1，建立急性免疫性肝损伤模型，16h后处理动物，制备血清，全自动生化分析仪检测血清A1JT，AST及LDH水平，H.E．染色考察肝脏病理状态。结果ConA20mg·kg-1能引起小鼠显著的急性免疫性肝损伤，血清ALT，AST及LDH含量均显著升高，肝组织出现以炎症细胞浸润、肝细胞坏死为主要特征的病理改变。注射用胡黄连总昔0．5～Cmg·kg-1剂量对ConA引起的肝损伤有显著保护作用，明显降低血清转氨酶水平，改善肝脏病理状态，其中1mg·kg-1剂量药效最佳，8mg·kg。剂量药效有所下降，但此剂量未显示明显毒性。结论注射用胡黄连总苷对ConA引起的免疫性肝损伤有明确的保护作用，其起效剂量低，高于8mg·kg-1活性下降，在临床实验时需注意剂量的选择问题。

Protective Effect of Total Glucoside of Picrorhiza Scrophulariflora for Injection on Concanavalin A-induced Liver Injury in Mice

Objective To study the protective effect of total glucoside o~Picrorhiza scrophulariflora（TGP） for infection on Concanavalin A（ConA）-induced acute liver injury in ICR mice. Methods ICR mice were given intravenously TGP injection 0.5- 8mg·kg-1 for five days（one time/day）. After the last administration 2h, the mice were injected intravenously ConA 20mg·kg-1 to establish the immunological liver damage model. All animals were killed on 16h atier ConA treatment. The activities of ALT, AST and LDH were measured by automatic chemistry analyzer（TBA-40FR）. Liver histopathological changes were examined by H.E. and light microscopy. Results ConA 20mg＇kg-1 could induced significant liver damage, expressed in the higher serum ALT,AST,LDH activities and the apoptosis or inflammatory cell infiltration in liver tissue. TGP injection 0.5 - 4mg·kg-1 showed significantly protective effect to hver damage induced by ConA, which could reduce serum transarninase activities and prevent the pathological injury. The optimum dose is lmg·kg-1. There was a reduced efficacy when the dose is 8mg·kg-1. While at this dose, there are no obvious toxicity. Conclusion TGP injection showed a significant protective effect on Con_A-induced liver injury in mice. Its effective dose is low and the therapeutic activity will cut down when the dose is higher than 8mg·kg-1. It should be carefi.fl to the dose choice in clinical trial.