| 反相HPLC-荧光法测定大白鼠组织及食物中α-二羧基类化合物的含量 |
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| 引用本文： | 朱萱萱,茅咏雯,何润霞,山口政俊. 反相HPLC-荧光法测定大白鼠组织及食物中α-二羧基类化合物的含量[J]. 中国生化药物杂志, 1997, 0(2) |
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| 作者姓名： | 朱萱萱 茅咏雯 何润霞 山口政俊 |
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| 作者单位： | 江苏省中医院临床研究所!南京，210029(朱萱萱)，江苏省职工医科大学(茅咏雯)，南京生化制药研究所(何润霞)，日本福冈大学药理分析研究室(山口政俊) |
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| 摘 要： | 采用最新合成的荧光试剂与大白鼠组织及食物中α-二羧基类化合物生成荧光诱导体，然后用反相HPLC－荧光法对大白鼠组织及食物中的乙二醛、甲基乙二醛、二乙酸和2，3－乙酸丙酮4种α-二羧基类化合物同时进行分离定量检出，色谱柱为L－ODS（100×8mmid），流动相为乙膊-0．5mol／L醋酸铵（35：65V／V），荧光检出波长为Ex350nmEm390nm，检出限度（S／N－3）11．6～13．8fmol／10ul，回收率均在90％以上。
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| 关 键 词： | 反相HPLC－荧光法 乙二醛 甲基乙二醛 二乙酰 2，3-乙酰丙酮 |
Determination of Glyoxal, Methylglyoxal, Diacethyl and 2, 3-Pentanedione in Rat Tissues and Foodstuffs by Liquid Chromatography with Fluorescence Detection |
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| Zhu Xuanxuan, Mao Yongwen, He Renxia, MASATOSHI YAMAGUCHI. Determination of Glyoxal, Methylglyoxal, Diacethyl and 2, 3-Pentanedione in Rat Tissues and Foodstuffs by Liquid Chromatography with Fluorescence Detection[J]. Chinese Journal of Biochemical Pharmaceutics, 1997, 0(2) |
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| Authors: | Zhu Xuanxuan Mao Yongwen He Renxia MASATOSHI YAMAGUCHI |
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| Affiliation: | Hospital of Traditional Chinese Medicine of Jiangsu Prouince Nanjing 210029 |
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| Abstract: | A highly sensitive and rapid liquid chromatograpraphic method for the determination of gly oxal, methlglyoxal, diacetyl and 2, 3-pentanedione in rat tissues and fdris is investigated. After ex traction of the compounds with methanol, the compounds in the extract are converted into the corre sponding fluorescent derivative by reaction with 1, 2-diamino- 4, 5-methylenedioxbenzene, a fluoro genic reagent for α-dicarbonyl compounds. The derivatives are separated on a reversed phase column with isocratic using acetonitrile-0. 5 mol/L ammonium acetate, and detected fluorimetrically. The de tection limits are 11. 6~ 13. 8 fmol/ 10μ1 injection for all the compounds at a signal to noise ratio of 3. |
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| Keywords: | Glyoxal Methylglyoxal Diacethyl 2 3-Pentanedione HPLC-fluorescence |
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