| Gastric cancer induced by N—methyl—N‘—nitro—N—nitrosoguanidine in rat with ulcers and expressions of ras and c—erbB2 genes |
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| 引用本文： | 周本杰,陈蔚文,等.Gastric cancer induced by N—methyl—N‘—nitro—N—nitrosoguanidine in rat with ulcers and expressions of ras and c—erbB2 genes[J].中国人民解放军军医大学学报,2003,18(1):8-10. |
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| 作者姓名： | 周本杰 陈蔚文 |
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| 作者单位： | [1]DepartmentofPharmacy,ZhujiangHospital,FirstMilitaryMedicalUniversity,Guangzhou510282,China [2]PiweiInstitute,GuangzhouUniversityofTraditionalChineseMedicine,Guangzhou510407,China |
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| 摘 要： | Objective:To observe the series of pathological changes during the development of gastric adenocarcinoma in ulcerative rats induced by N-methyl-N‘-nitrosoguanidine(MNNG),and the expression profile of related oncogenic protein.Methods:MNNG was administered in rats with ulcers due to acetic acid treatment to induce gastric cancer,and the protein expressions of ras and c-erbB2 genes in the ulcer were examined immunohistochemically along with pathological examination.Results:The incidence of gastric adenocarcinoma in the model group reaches 40%(6/15),while none of the rats developed cancer in the control group with ulcers.Postiive expressions of the proteins of p21 ras and c-erbB2 were observed in the tissues undergoing canceration in the 6 rats of model group,but were not observed in the 5 control rats;p53 protein expression,however,failed to be detected in both groups.Conclusion:A new animal model of gastric cancer has been established in rats with gastric ulcer after MNNG treatment,which may facilitate the pharmacological reserach of gastric cancer.
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| 关 键 词： | 胃癌 大鼠 动物模型 MNNG ras c－erbB2 溃疡 基因表达 亚硝基化合物 |
Gastric cancer induced by N-methyl-N'-nitro-N-nitrosoguanidine in rat with ulcers and expressions of ras and c-erbB2 genes |
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| ZHOU Beng-jie,CHEN Wei-wen,XU Qin LI Ru-liu,WANG Jian-hua.Gastric cancer induced by N-methyl-N''''-nitro-N-nitrosoguanidine in rat with ulcers and expressions of ras and c-erbB2 genes[J].Journal of Medical Colleges of PLA(China),2003,18(1):8-10. |
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| Authors: | ZHOU Beng-jie CHEN Wei-wen XU Qin LI Ru-liu WANG Jian-hua |
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| Institution: | ZHOU Beng-jie,CHEN Wei-wen,XU Qin LI Ru-liu,WANG Jian-hua
Department of Pharmacy,Zhujiang Hospital,First Military Medical University,Guangzhou 510282,China Piwei Institute,Guangzhou University of Traditional Chinese Medicine,Guangzhou 510407,China |
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| Abstract: | Objective: To observe the series of pathological changes during the development of gastric adenocarcinoma in ulcerative rats induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) , and the expression profile of related on-cogenic protein. Methods: MNNG was administered in rats with ulcers due to acetic acid treatment to induce gastric cancer, and the protein expressions of ras and c-erbB2 genes in the ulcer were examined immunohistochemically along with pathological examination. Results: The incidence of gastric adenocarcinoma in the model group reaches 40% (6/ 15) , while none of the rats developed cancer in the control group with ulcers. Positive expressions of the proteins of p21ras and c-erbB2 were observed in the tissues undergoing canceration in the 6 rats of model group, but were not observed in the 5 control rats; p53 protein expression, however, failed to be detected in both groups. Conclusion: A new animal model of gastric cancer has been established in rats with gastric ulcer after MNNG treatment, which may facilitate the pharmacological research of gastric cancer. |
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| Keywords: | gastric cancer animals' disease model MNNG oncogenic protein expression |
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