三氧化二砷诱导人胰腺癌细胞凋亡的实验研究

目的：研究三氧化二砷（As2O3)体外抑制人胰腺癌细胞株SW1990增殖及诱导其凋亡的作用。方法：以不同浓度的As2O3处理SW1990，用MTT法测定细胞增殖情况，并利用AnnexinⅤ早期凋亡检测试剂盒、电子显微镜、流式细胞仪以及免疫组化染色等检测细胞凋亡情况。结果：（1）As2O3抑制SW1990细胞增殖的作用与相同浓度的顺铂相似。（2）10ug/mlAs2O3作用12h后即观察到细胞凋亡明显增多，48h后凋亡率达24%；免疫组化染色证实As2O3作用后细胞Bcl-2表达阳性率较作用前及对照组减少，Bcl-2/Bax比值耳降。结论：As2O3体外可抑制SW1990增殖，其主要途径是诱导细胞凋亡。

Experimental study on arsenic trioxide-induced apoptosis of human pan creatic cancer cell line

Objective: To study the effect of arsenic trioxide (As2O3) on human pancreatic cancer cell proliferation and apoptosis (mainly early stage) in vitro. Methods: SW1990 cells line were trea ted with As2O3 at different concentration. Cell proliferation was evaluated by MTT and apoptosis by Annexin-Ⅴ-fluostaining, electron-microscopy, flow cytometry and immunocytochemical staining of Bcl-2 and Bax. Results: As2O3 and cisplatin had the same cytotoxity on SW1990. The cytotoxic effe ct on tumor cell was produced by induction of apoptosis. Twelve hours after cult ure with 10 μg/ml As2O3, much more SW1990 cells went into apoptosis than t he control. The apoptosis rate reached 24% after 48 h with the similar concentra tion of As2O3. Immunohistochemical study revealed that the expression of Bcl -2 was decreased after treated with As2O3. Conclusion: As 2O3 can depress the proliferation of SW1990 in vitro, mainly through the i nduction of apoptosis, and it is a potential agent for pancreatic cancer chemoth erapy.