我国嗜人按蚊不同地理株基因鉴别的初步研究

目的　分析不同地区嗜人按蚊的基因特征。　方法　选择 13种随机引物对嗜人按蚊江苏、广西和四川实验株以及从湖北和广东现场捕获的嗜人按蚊进行随机引物 PCR扩增 ,并以中华按蚊江苏实验株为对照。　结果　 13种随机引物中有 7种随机引物对中华按蚊和 5个地区的嗜人按蚊 PCR扩增的结果存在差别。S-随机引物对中华按蚊和不同地区嗜人按蚊的扩增结果出现明显差异。中华按蚊和不同地区的嗜人按蚊均出现 4 5 0 bp较强扩增条带。但 2 4 0 bp的强扩增条带为中华按蚊所特有。嗜人按蚊江苏和四川实验株分别在 2 30 bp和 32 0 bp处有 1条强扩增条带 ,而从湖北和广东现场采集的嗜人按蚊分别在 4 0 0 bp和 2 6 0 bp处出现较强的扩增条带。　结论　采用随机引物 PCR技术对不同地区嗜人按蚊的基因分析已显示明显差异。已发现的一些特有的 DNA扩增条带可被用作进一步的基因特征分析和鉴别。

A PRELIMINARY STUDY ON GENETIC IDENTIFICATION OF ISOLATES OF ANOPHELES ANTHROPOPHAGUS IN CHINA

Objective To analyze the genetic characteristics of Anopheles anthropophagus from different areas. Methods A random primer polymerase chain reaction with thirteen selected primers was applied to identify Jiangsu, Guangxi and Sichuan laboratory isolates of An. anthropophagus and field collected An. anthropophagus from Guangdong and Hubei Provinces. The Jiangsu laboratory isolate of An. sinensis was used as control. Results Amplified with thirteen primers, seven of them showed the difference in different laboratory isolates and field collected An. anthropophagus . The S random primer showed remarkable difference amplified DNA band patterns between An. sinensis and An. anthropophagus from different areas. There is a 450 bp DNA band amplified in all of tested An. sinensis and An. anthropophagus samples but a 240 bp DNA band is only showed in An. sinensis . However, there are a 320 bp,230 bp,400 bp and 260 bp fragments amplified in Sichuan and Jiangsu laboratory isolates of An. anthropophagus as well as in the field collected An. anthropophagus from Hubei and Guangdong Provinces respectively. Conclusion RAPD showed the differences among the Jiangsu, Guangxi and Sichuan laboratory isolates of An. anthropophagus as well as Guangdong and Hubei field collected An. anthropophagus . Some amplified specific DNA fragments can be used for further genetic identification of isolates of An. anthropophagus .