正交试验优选四逆汤机械煎煮工艺

目的:优化四逆汤机械煎煮工艺。方法:以苯甲酰新乌头原碱、甘草苷、甘草酸和6-姜酚的含量为考核指标,采用RP-HPLC法测定指标成分含量,通过单因素试验和正交设计考察加水量、浸泡时间、煎煮时间及煎煮次数等影响因素,优选四逆汤机械煎煮工艺。结果:四逆汤最佳机械煎煮工艺为浸泡时间60 min,煎煮时间60 min,加水量10倍,煎煮2次。结论:优化的煎煮工艺合理可行,为提高四逆汤剂质量与临床疗效提供实验基础。

Optimization of Mechanical Boiling Technology for Sini Decoction by Orthogonal Test

Objective:To optimize mechanical boiling technology of Sini decoction. Method: With the contents of benzoylmesaconine, liquiritin, glycyrrhizic acid and 6-gingerol as indexes,the content of indicator ingredients were determined by HPLC,mechanical boiling technology of Sini decoction was optimized by single factor test and orthogonal test with the amount of water,soaking time,boiling time and boiling times as factors. Result: Optimum mechanical boiling technology was as follows:soaked 60 min with 10 times the amount of water,boiled 2 times with 60 min per time. Conclusion: This optimized technology was rational and feasible, it could provide experimental basis for improving quality and clinical efficacy of Sini decoction.