WT1基因在白血病中的表达及临床意义

目的　探讨WT1基因在白血病中的作用。方法　用定量RT -PCR方法检测病人外周血或骨髓单个核细胞WT1mRNA表达。结果　 5 0例白血病患者中 ,41例WT1mRNA表达增高 ,阳性率为 82 0 %。急性髓细胞白血病 (AML)和急性淋巴细胞白血病 (ALL)阳性率分别为 84 0 %和 93 8% ,两组无显著差异 (P >0 0 5 ) ,慢性粒细胞白血病 (CML)的WT1mRNA阳性率为 6 6 7% ,其中慢性期均阴性 ,加速期和急变期均阳性。 17例完全缓解患者中 ,15例 (85 2 % )WT1mRNA转阴 ,复发患者WT1mRNA再次升高。结论　WT1mRNA表达的测定对白血病微小残留病检测具有重要意义

Clinical significance of Wilms' gene(WT1) expression in patients with leukemia

Objective　To evaluate the role of WT1 gene in leukemia.Methods　The expression of WT1 mRNA in peripheral blood or bone marrow cells was detected by RT-PCR method.Results　The expression of WT1 mRNA increased in 41 of 50 patients with leukemia，and the positive rate was 82.0%. The WT1 mRNA positive rates in acute myeloid leukemias and acute lymphoblastic leukemias were 84.0% and 93.8%,respectively，and there was no significant difference between the two groups(P>0.05).The WT1 positive rate in chronic myeloid leukemia was 66.7%，in which the WT1 was negative in patients at chronic phase，but positive in both accelerated phase and blast crisis.WT1 mRNA became negative in 15 of 17(85.2%) patients achieving complete remission，but it increased again in the relapsed patients.Conclusion　WT1 mRNA is an important molecular marker for detection of minimal residual disease in leukemia.