巨结肠类缘病病理形态学诊断的探讨

目的采用NSE和S-100蛋白免疫组化染色方法,探讨巨结肠类缘病的病理形态学特征,提高其诊断率。方法对97例行巨结肠根治术后的病变肠段进行常规HE染色,并对其中21例采用NSE和S-100蛋白免疫组化,观察肠神经元及神经节细胞形态和数量的变化并与正常组进行比较。结果巨结肠类缘病(alliedHD)标本的神经元与神经节细胞数与正常组及巨结肠症(HD)比较差异有显著性(P<0.01)。结论巨结肠类缘病的诊断主要根据病理特征而定,在HE染色基础上结合NSE及S-100蛋白免疫组织化学染色能提高确诊率。

Investigation on the pathological morphology for Diagnosis of allied HD

Objective To study the pathomorphological characteristics of allied Hirschsprung's dis ease(allied HD)by using neuron specific enolose (NSE)and S-100protein immunohistochemical stain ing techniques,in order to improve the early diagnosis rates.Methods 97cases with HD were in cluded in this study,the morphological and numerical change of the neurone and ganglion cell lesions were observed by routine HE staining,and compared with the normal controls.NSE and S-100pro-tein immunohistochemical staining were carried out in21cases.Results The number of the neurone and ganglion cell of allied HD was significantly higher than those in the normal control or HD group(P<0.01).Conclusions The diagnosis of allied HD is depended mainly on its pathologic characteris-tics.In combination of NSE and S-100protein immunohistochemical markers staining and routine HE stain ing have greatly helpful to promote a definite diagnosis.