黄芪多糖与总黄酮抗小鼠四环素肝毒性的比较研究

目的 比较黄芪多糖与总黄酮对四环素所致小鼠肝毒性的保护作用.方法 将ICR小鼠分为5组,给药组分别给予黄芪多糖（500 mg/kg）、总黄酮（500 mg/kg）和联苯双酯（150 mg/kg）,各组连续灌胃相应药物10 d,末次给药1h后腹腔注射四环素200 mg/kg进行造模.24h后利用生化法测定血清ALT、AST、TG和CHO,肝组织TG和CHO水平,以及MDA、SOD、GSH的含量;采用HE染色法检测肝组织病理组织学改变.结果 黄芪多糖可抑制四环素所致小鼠血清ALT、AST和肝组织CHO的升高,以及血清TG和CHO的下降（P＜0.01,P＜0.05）;而总黄酮只抑制血清ALT和肝组织CHO的异常升高（P＜0.01）,对血清AST、TG、CHO以及肝组织TG无明显作用,但可进一步升高GSH水平（P＜0.05）.且二者均可明显减轻肝细胞脂肪变性、水肿及坏死.结论 黄芪多糖和总黄酮对四环素所致小鼠肝毒性具有明显的保护作用,多糖作用明显强于总黄酮,其机制可能与抗氧化作用相关.

Comparative Study of Astragalus Polysaccharide and Total Flavonoids against Tetracycline Induced Liver Toxicity in Mice

Objective To investigate the comparative effect of astragalus polysaccharide and total flavonoids on liver toxicity induced by tetracycline. Methods The ICR mice were divided into five groups. The drug groups were given orally with corresponding polysaccharide （500 mg/kg）, total flavonoids （500 mg/kg） and bifendate （150 mg/kg） for 10 consecutive days as positive controlwere given orally to mice once a day, respectively. Tetracycline （200 mg/kg, intraperitoneally） was injected at 1 h after the last administration to build models. After 24 h, the level of serum ALT, AST, TG and CHO; TG and CHO level in liver tissue; and the contents of MDA, SOD and GSH were assayed by biochemistry. The liver histopathology lesion was detected by HE stain. Results Astragalus polysaccharide significantly inhibited the increase of serum ALT and AST, and CHO in livertissue, inhibited the decreasing of serum TG and CHO （P〈0.01, P〈0.05）. However, total flavonoids only inhibited the increase of serum ALT and hepatic CHO （P〈0.01） and did not show obvious effect on AST, TG and CHO in liver tissues, while can increase GSH level （P〈0.05）. Additionally, astragalus polysaccharide and total flavonoids markedly attenuated hepatic steatosis, vacuolar degeneration and necrosis. Conclusion Astragalus polysaccharide and total flavonoids showed a significant protective effect on hepatic damage and steatosis induced by tetracycline. But the effect of astragalus polysaccharide is significantly stronger than total flavonoids. The mechanism may be related with anti-oxidation effect.