中国海域分离的啮氏艾肯菌致病性和抗生素敏感性研究

目的 就中国海域分离的啮氏艾肯菌对小鼠的毒力及伤口感染情况进行研究,并进行抗生素敏感性实验.方法 昆明小鼠腹腔注射啮氏艾肯菌悬液,观察一般状态、白细胞计数和血培养.第5天脱颈处死后解剖取脏器甲醛固定,石蜡包埋、制片,HE染色,光镜观察;昆明小鼠致伤后浸泡在含啮氏艾肯菌的人工海水中45～60 min,打捞后分笼于层流柜中饲养.于致伤浸泡后不同时间做白细胞计数及血培养,并观察一般状态和伤口炎性反应,定时取伤肢分泌物做细菌培养;实验完毕后伤肢甲醛同定,石蜡包埋、制片,HE染色,光镜检查;用K-B法做啮氏艾肯菌对各种抗生素药物敏感性实验.结果 小鼠注射啮氏艾肯菌液后,白细胞升高,感染12 h后20%小鼠心和肝脏有细菌生长.小鼠感染啮氏艾肯菌后,病变部位以肺、肝及.肾脏为重,病变性质主要是造成血液循环障碍,引起不同程度的充血、出血及血停滞现象.致伤小鼠经含啮氏艾肯菌的海水浸泡后,6、12、24、48、72 h伤肢培养均为阳性,感染后48 h时20%小鼠肠组织细菌培养阳性,其余时间点和其他组织细菌培养均为阴性.伤肢组织可见肌纤维排列疏松、紊乱、断裂,横纹肌间较多炎细胞浸润.啮氏艾肯菌对复方新诺明、哌拉西林、美洛培南、磺胺的敏感性仅为67%,对其他抗生素均表现为敏感.结论 中国海域分离的啮氏艾肯菌具有致病力,啮氏艾肯菌对复方新诺明、哌拉西林、美洛培南、磺胺的敏感性较低,对其他抗生素均表现为敏感.

Pathogenicity and antibiotic sensitivity of Eikenella corrodens isolated from maritime space of China

Objective To study the toxicity, wound infection ability and antibiotic sensitivity of Eikenella corrodens isolated from maritime space of China. Methods Kunming mice were intraperitoneally injected with Eikenella corrodens suspension, and their general status was observed and leukocyte count and blood culture were preformed. On the fifth day after infection, mice were sacrificed and their organs were treated with formalin fixation, paraffin imbedding, HE staining and observed under light microscope. In tox...