乙型肝炎患者血清中分泌型IgA水平与HBV DNA含量的关系

湖北省鹤峰县人民医院检验科,鹤峰 445800目的 研究乙型肝炎患者血清中分泌型IgA(SIgA)水平与乙型肝炎病毒(HBV)DNA含量的关系,为临床提供一个新的评价HBV复制状态及肝细胞损伤的指标。方法 100份经ELISA检测并确诊为乙型肝炎的患者血清用荧光定量PCR检测HBV DNA的拷贝数,用ELISA并经酶标仪定量其SIgA的量。结果 SIgA的含量与HBVDNA的拷贝数的对数呈正相关(r=0.69,P<0.01),在HBsAg( )/HBeAg( )/HBcAb( )组和HBsAg( )/HBeAb( )/HBcAb( )组中SIgA含量差异无显著性意义(P>0.05),而HBV DNA拷贝数前者明显高于后者(P<0.01)。结论 乙型肝炎患者血清SIgA的含量在评价HBV的传染性及肝细胞损伤程度方面比乙型肝炎血清学标志物(HBV-M)更灵敏、准确。

Relationship between Serum HBV DNA Copies and SIgA Level in Hepatitis B Patients

Objective To evaluate the relationship between serum hepatitis B virus (HBV) DNA copies and SIgA level in hepatitis B patients. Methods A series of serum samples from 100 patients who were diagnosed as having hepatitis B were analyzed. The serum HBV DNA copies were tested by the fluorescence quantitative polymerase chain reaction using the Roche Lighter Cycler. The serum SIgA level was quantitated by ELISA using photometer at 405 nm. Results There was a logarithmic correlation between HBV DNA copies and serum SIgA level (r=0. 692, P<0. 01), and the HBV DNA copies were much higher in HBsAg ( ) /HBeAg ( ) /HBcAb ( ) samples than in HBsAg ( ) /HBeAb ( ) /HB-cAb ( ) samples, but no obvious difference in SIgA level was found between them (P>0. 05). Conclusion Serum SIgA in hepatitis B patients is an important marker in evaluating the infectiousness of HBV and the degree of hepatocyte necrosis.