| 阴囊升温对大鼠睾丸生精细胞凋亡及增殖细胞核抗原表达的影响 |
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| 引用本文： | 许多,秦达念,王勇,庄楚香. 阴囊升温对大鼠睾丸生精细胞凋亡及增殖细胞核抗原表达的影响[J]. 生殖医学杂志, 2003, 12(2): 80-84 |
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| 作者姓名： | 许多 秦达念 王勇 庄楚香 |
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| 作者单位： | 汕头大学医学院生理教研室,广东汕头,515031 |
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| 基金项目： | 广东省自然科学基金项目 (0 2 2 2 72 )，汕头大学研究与发展基金项目 (L 0 0 0 0 3) |
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| 摘 要： | 目的 研究阴囊升温对大鼠睾丸生精细胞凋亡及增殖细胞核抗原 ( PCNA)表达的影响 ,探讨其在升温引起生精细胞变化中的意义。 方法 应用原位末端标记( TUNEL )法和免疫组化法 ,检测大鼠睾丸局部升温至 43℃、3 0 min后 ,经 0 .5、1、3、6、1 0和 50 d观察生精细胞凋亡和 PCNA表达情况。 结果 大鼠睾丸局部升温至 43℃、3 0min后生精上皮受到损伤 ,生精细胞凋亡增加 ( P<0 .0 1 ) ,生精细胞 PCNA表达下降( 0 .5～ 1 0 d组 P<0 .0 1 ,50 d组 P<0 .0 5)。 结论 43℃、3 0 min作用后生精细胞 PCNA表达与细胞凋亡呈负相关 ,联合检测两者对评估热作用后生精功能的变化有重要意义
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| 关 键 词： | 阴囊升温 生精细胞 凋亡 增殖细胞核抗原 |
| 文章编号： | 1004-3845(2003)02-0080-05 |
| 修稿时间： | 2002-10-14 |
Significance of hyperthermic treatment on apoptosis and proliferating cell nuclear antigen expression in rat germ cell |
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| XU Duo,QIN Da nian,WANG Yong,ZHUANG Chu xiang. Significance of hyperthermic treatment on apoptosis and proliferating cell nuclear antigen expression in rat germ cell[J]. Journal of Reproductive Medicine, 2003, 12(2): 80-84 |
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| Authors: | XU Duo QIN Da nian WANG Yong ZHUANG Chu xiang |
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| Affiliation: | XU Duo,QIN Da nian,WANG Yong,ZHUANG Chu xiang Department of Physiology,Medical College of Shantou University,Shantou,Guangdong 515031 |
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| Abstract: | Objective: To explore the significance of hyperthermic treatment on apoptosis and proliferating cell nuclear antigen (PCNA) expression in rat germ cell. Methods: Germ cell apoptosis and PCNA expression were detected on day 0.5,1,3,6,10 and 50 after heat exposure (43℃ 30 min) by means of TdT mediated dUDP X nick end labeling(TUNEL) and immunohistochemistry. Results: After hyperthermic treatment, seminiferous epithelium was damaged. Germ cell apoptosis was increased ( P<0.01 ) and PCNA expression was decreased( 0.5~ 10 d: P<0.01 ;50 d: P<0.05 ). Conclusion: There was a negative correlation betweeen PCNA expression and apoptosis in rat germ cell after hyperthermic treatment. The combined determination plays an important role in the evaluation of hyperthermic treatment on spermatogenesis. |
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| Keywords: | Hyperthermic treatment Germ cell Apoptosis Proliferative cell nuclear antigen |
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