CD16在阵发性睡眠性血红蛋白尿症中的特异性表达

目的:检测中性粒细胞表面分化抗原CD16在阵发性睡眠性血红蛋白尿症(PNH)中表达,评价其在PNH诊断中的意义。方法:运用流式细胞术检测40名健康体检者及36例初治PNH患者外周血中性粒细胞表面分化抗原CD16,并与CD55、CD59及与传统Ham's试验结果进行比较研究。结果:PNH患者外周血细胞的CD16、CD55及CD59均有不同程度的缺乏,与健康对照组比较,差异有统计学意义(P<0.01);与传统Ham's试验比较其灵敏度高,特异度高。结论:细胞膜上糖基化磷脂酰肌醇锚的锚定蛋白CD16的缺陷与PNH有密切关系,流式细胞术检测PNH患者外周血中性粒细胞CD16可作为诊断PNH的有效辅助检查方法,具有一定临床意义。

Specific expression of CD16 on neutrophils in patients with paroxysmal nocturnal hemoglobinuria

Objective：To investigate the expression of CD16 on neutrophils in paroxysmal nocturnal hemoglobinuria（PNH） and evaluate its significance.Methods：The CD16 expression of peripheral blood neutrophils from forty healthy blood donors as control and thirty-six cases with PNH were measured by flow cytometry.The comparative study was carried on with CD55,CD59 and traditional Ham′s test.Results：Compared with normal controls,the expressions of CD16,CD55 and CD59 in patients with PNH were deficient at different degrees,the difference was of statistical significance（P0.01）.Its accuracy was more high and specific than Ham′s test.Conclusions：PNH is related with a deficiency of glycosylphosphatidylinositol-anchored proteins CD16.CD16 assay on peripheral blood neutrophils by flow cytometry is a sensitive and specific method for PNH diagnosis and it may have an important clinical significance for diagnosis of PNH patients.